Document Detail


Study of IspH, a key enzyme in the methylerythritol phosphate pathway using fluoro-substituted substrate analogues.
MedLine Citation:
PMID:  21981393     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
IspH, a [4Fe-4S]-cluster-containing enzyme, catalyzes the reductive dehydroxylation of 4-hydroxy-3-methyl-butenyl diphosphate (HMBPP) to isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) in the methylerythritol phosphate pathway. Studies of IspH using fluoro-substituted substrate analogues to dissect the contributions of several factors to IspH catalysis, including the coordination of the HMBPP C(4)-OH group to the iron-sulfur cluster, the H-bonding network in the active site, and the electronic properties of the substrates, are reported.
Authors:
Youli Xiao; Wei-chen Chang; Hung-wen Liu; Pinghua Liu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-10-07
Journal Detail:
Title:  Organic letters     Volume:  13     ISSN:  1523-7052     ISO Abbreviation:  Org. Lett.     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-10-28     Completed Date:  2012-01-18     Revised Date:  2013-10-31    
Medline Journal Info:
Nlm Unique ID:  100890393     Medline TA:  Org Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5912-5     Citation Subset:  IM    
Copyright Information:
© 2011 American Chemical Society
Affiliation:
Department of Chemistry, Boston University, Boston, Massachusetts 02215, USA.
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MeSH Terms
Descriptor/Qualifier:
Alkyl and Aryl Transferases / metabolism*
Biocatalysis
Erythritol / chemistry*,  metabolism
Fluorine Compounds / chemistry*,  metabolism
Models, Molecular
Molecular Structure
Oxidation-Reduction
Substrate Specificity
Sugar Phosphates / chemistry*,  metabolism
Grant Support
ID/Acronym/Agency:
GM040541/GM/NIGMS NIH HHS; GM093903/GM/NIGMS NIH HHS; R01 GM040541-22/GM/NIGMS NIH HHS; R01 GM093903/GM/NIGMS NIH HHS; R01 GM093903-03/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorine Compounds; 0/Sugar Phosphates; 149-32-6/Erythritol; EC 2.5.-/Alkyl and Aryl Transferases
Comments/Corrections

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