Document Detail


Study of Helicobacter pylori genotype status in saliva, dental plaques, stool and gastric biopsy samples.
MedLine Citation:
PMID:  22563199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To compare genotype of Helicobacter pylori (H. pylori) isolated from saliva, dental plaques, gastric biopsy, and stool of each patient in order to evaluate the mode of transmission of H. pylori infection.
METHODS: This cross-sectional descriptive study was performed on 300 antral gastric biopsy, saliva, dental plaque and stool samples which were obtained from patients undergoing upper gastrointestinal tract endoscopy referred to endoscopy centre of Hajar hospital of Shahrekord, Iran from March 2010 to February 2011. Initially, H. pylori strains were identified by rapid urease test (RUT) and polymerase chain reaction (PCR) were applied to determine the presence of H. pylori (ureC) and for genotyping of voculating cytotoxin gene A (vacA) and cytotoxin associated gene A (cagA) genes in each specimen. Finally the data were analyzed by using statistical formulas such as Chi-square and Fisher's exact tests to find any significant relationship between these genes and patient's diseases. P < 0.05 was considered statistically significant.
RESULTS: Of 300 gastric biopsy samples, 77.66% were confirmed to be H. pylori positive by PCR assay while this bacterium were detected in 10.72% of saliva, 71.67% of stool samples. We were not able to find it in dental plaque specimens. The prevalence of H. pylori was 90.47% among patients with peptic ulcer disease (PUD), 80% among patients with gastric cancer, and 74.13% among patients with none ulcer dyspepsia (NUD) by PCR assay. The evaluation of vacA and cagA genes showed 6 differences between gastric biopsy and saliva specimens and 11 differences between gastric and stool specimens. 94.42% of H. pylori positive specimens were cagA positive and all samples had amplified band both for vacA s and m regions. There was significant relationship between vacA s1a/m1a and PUD diseases (P = 0.04), s2/m2 genotype and NUD diseases (P = 0.05). No statically significant relationship was found between cagA status with clinical outcomes and vacA genotypes (P = 0.65). The evaluation of vacA and cagA genes showed 6 differences between gastric biopsy and saliva specimens and 11 differences between gastric and stool specimens.
CONCLUSION: Regard to high similarity in genotype of H. pylori isolates from saliva, stomach and stool, this study support the idea which fecal- oral is the main route of H. pylori transmission and oral cavity may serve as a reservoir for H. pylori, however, remarkable genotype diversity among stomach, saliva and stool samples showed that more than one H. pylori genotype may exist in a same patient.
Authors:
Hassan Momtaz; Negar Souod; Hossein Dabiri; Meysam Sarshar
Related Documents :
15902109 - Duration of human chorionic gonadotropin surveillance for partial hydatidiform moles.
2455739 - Sonographic investigation of the uterus during artificial uterine cavity distention.
2883139 - The efficacy of progesterone in achieving successful pregnancy: i. prophylactic use dur...
50569 - Visceral radioisotopic lymphography of the testicle.
22842299 - Long-term cyclic intravenous iloprost in systemic sclerosis: clinical experience from a...
6476229 - Negative findings at appendectomy.
23821509 - Neutrophilic reversible allograft dysfunction (nrad) and restrictive allograft syndrome...
15332419 - Research methodology: cancer cachexia syndrome.
404029 - Asynchronous development of two monoclonal proteins (igm lambda and delta 1 chains) in ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  18     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-07     Completed Date:  2012-09-18     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  2105-11     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antigens, Bacterial / genetics
Bacterial Proteins / genetics
Biopsy
Cross-Sectional Studies
Dental Plaque / microbiology*
Feces / microbiology*
Female
Genotype
Helicobacter pylori / classification*,  genetics
Humans
Male
Middle Aged
Saliva / microbiology*
Stomach / microbiology*,  pathology
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/VacA protein, Helicobacter pylori; 0/cagA protein, Helicobacter pylori
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differences between diffuse and focal autoimmune pancreatitis.
Next Document:  Association of NOD1 and NOD2 genes polymorphisms with Helicobacter pylori related gastric cancer in ...