Document Detail


Study on CXCR4/SDF-1alpha axis in lymph node metastasis of cervical squamous cell carcinoma.
MedLine Citation:
PMID:  17362322     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
CXCR4/stromal-cell-derived factor-1alpha (SDF-1alpha) is involved in many cancer metastatic mechanisms. Cervical squamous cell cancer (SCC) tissues (n=35), normal cervical tissues (n=10), metastatic (n=10) and nonmetastatic lymph nodes (n=50), and Hela cells were stained immunohistochemically with CXCR4 monoclonal antibody (mAb). Meanwhile, lymph nodes were stained immunohistochemically with rabbit anti-SDF-1alpha. In vitro invasion of Hela cells was evaluated using Transwell Permeable Supports (Corning, NY), in which Hela cells with/without CXCR4 mAb preincubation were seeded in the upper chambers and medium containing 0-100 ng/mL SDF-1alpha was added to the lower compartments. For evaluating the effect of CXCR4/SDF-1alpha on proliferation of cervical cancer cells, Hela cells were cultured for 72 h exposed to SDF-1alpha with and without CXCR4 mAb. We found that CXCR4 was expressed on SCC cells in all cervical cancer, metastatic lymph node, and Hela cells but not in normal cervix. SDF-1alpha was expressed on lymph cells in all lymph nodes. SDF-1alpha induced the directed migration of Hela cells with a concentration-dependent model, which was inhibited by CXCR4 mAb (P<0.05). SDF-1alpha also stimulated the proliferation of Hela cells mediated by CXCR4 (P<0.05). CXCR4/SDF-1alpha axis probably participates in the metastasis toward lymph nodes in cervical cancer.
Authors:
J-P Zhang; W-G Lu; F Ye; H-Z Chen; C-Y Zhou; X Xie
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of gynecological cancer : official journal of the International Gynecological Cancer Society     Volume:  17     ISSN:  1048-891X     ISO Abbreviation:  Int. J. Gynecol. Cancer     Publication Date:    2007 Mar-Apr
Date Detail:
Created Date:  2007-03-16     Completed Date:  2007-05-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9111626     Medline TA:  Int J Gynecol Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  478-83     Citation Subset:  IM    
Affiliation:
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Antibodies, Monoclonal / pharmacology
Carcinoma, Squamous Cell / metabolism,  pathology*
Cell Movement / drug effects
Cell Proliferation / drug effects
Chemokine CXCL12
Chemokines, CXC / metabolism,  pharmacology*
Female
Hela Cells
Humans
Immunohistochemistry
Lymphatic Metastasis / pathology
Middle Aged
Receptors, CXCR4 / antagonists & inhibitors*,  metabolism
Uterine Cervical Neoplasms / metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/CXCL12 protein, human; 0/Chemokine CXCL12; 0/Chemokines, CXC; 0/Receptors, CXCR4

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