| Studies with bioengineered Nisin peptides highlight the broad-spectrum potency of Nisin V. | |
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MedLine Citation:
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PMID: 21255345 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Nisin A is the most thoroughly investigated member of the lantibiotic family of antimicrobial peptides. In addition to a long history of safe use as a food antimicrobial, its activity against multi-drug resistant pathogens has resulted in a renewed interest in applying nisin as a chemotherapeutic to treat bacterial infections. The wealth of Nisin-related information that has been generated has also led to the development of the biotechnological capacity to engineer novel Nisin variants with a view to improving the function and physicochemical properties of this already potent peptide. However, the identification of bioengineered Nisin derivatives with enhanced antimicrobial activity against Gram-positive targets is a recent event. In this study, we created stable producers of the most promising derivatives of Nisin A generated to date [M21V (hereafter Nisin V) and K22T (hereafter Nisin T)] and assessed their potency against a range of drug-resistant clinical, veterinary and food pathogens. Nisin T exhibited increased activity against all veterinary isolates, including streptococci and staphylococci, and against a number of multi-drug resistant clinical isolates including MRSA, but not vancomycin-resistant enterococci. In contrast, Nisin V displayed increased potency against all targets tested including hVISA strains and the hyper-virulent Clostridium difficile ribotype 027 and against important food pathogens such as Listeria monocytogenes and Bacillus cereus. Significantly, this enhanced activity was validated in a model food system against L. monocytogenes. We conclude that Nisin V possesses significant potential as a novel preservative or chemotherapeutic compound. |
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Authors:
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Des Field; Lisa Quigley; Paula M O'Connor; Mary C Rea; Karen Daly; Paul D Cotter; Colin Hill; R Paul Ross |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbial biotechnology Volume: 3 ISSN: 1751-7915 ISO Abbreviation: Microb Biotechnol Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2011-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101316335 Medline TA: Microb Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 473-86 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd. |
Affiliation:
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Department of Microbiology, University College Cork, Cork, Ireland. Teagasc, Moorepark Food Research Centre, Fermoy, Co. Cork, Ireland. Alimentary Pharmabiotic Centre, University College Cork, Ireland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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