Document Detail

Studies on the ultrastructure of blood cells and the microaggregate fraction in stored human blood.
MedLine Citation:
PMID:  7217514     Owner:  NLM     Status:  MEDLINE    
Electron microscopy of citrate-phosphate-dextrose (CPD) buffered bank blood performed over 21 days shows that the normal architecture of erythrocytes, platelets and leucocytes disappears and that deformed organelles, leucocyte "ghosts", cell fragments and microaggregates accumulate. Leucocytes and platelets emerge as the most sensitive indicators of blood deterioration showing profound morphological changes from the first day. Microaggregates which passed through a 20-micrometer high capacity transfusion filter were identified as platelet conglomerates, leucocyte "ghost" and platelet-leucocyte "ghost" coaggregates with diameters of 6--20, 20--30 and 30--40 micrometers respectively. Of these aggregates present in the filtrate, 62% fell into the size range of 20--40 micrometers. The composition of microaggregates varies with storage time, the platelet conglomerates appear first during or after Day 1, leucocyte "ghosts" after Day 5 followed by platelet-leucocyte "ghost" coaggregate formation. At this stage the number of intact leucocytes and platelets is reduced and the filtrate shows an abundance of leucocyte debris. Microfiltration would thus appear to reduce but not eliminate the danger of microembolism and damage to capillary endothelium.
E J Truter; J J Rossouw; L Böhm
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Intensive care medicine     Volume:  7     ISSN:  0342-4642     ISO Abbreviation:  Intensive Care Med     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-06-25     Completed Date:  1981-06-25     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7704851     Medline TA:  Intensive Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  115-9     Citation Subset:  IM    
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MeSH Terms
Blood Cells / ultrastructure*
Blood Platelets / ultrastructure
Blood Preservation
Erythrocytes / ultrastructure
Leukocytes / ultrastructure
Microscopy, Electron
Platelet Aggregation
Time Factors

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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