Document Detail


Studies on the turnover rates of ornithine aminotransferase in Morris hepatoma 44 and host liver.
MedLine Citation:
PMID:  187580     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ornithine aminotransferase [EC 2.6.1.13] content of Morris hepatoma 44 is about 15 times higher than that in normal liver. The turnover rates of ornithine amino-transferase in hepatoma 44 and host liver were determined using L-[14C]leucine. Studies on the incorporation of radioactive leucine into ornithine aminotransferase in rats bearing hepatoma 44 showed that the rate of synthesis of this enzyme in the hepatoma was about 5-fold higher than that in host liver. The half-life of ornithine aminotransferase in host liver was 0.98 day, which was the same as that in normal liver, whereas that in hepatoma 44 was 3.5 days. The rate constant of degradation of ornithine aminotransferase in hepatoma 44 was significantly less than that in host liver. These results show that the high ornithine aminotransferase content of hepatoma 44 is due to both increase in its rate of synthesis and decrease in its rate of degradation.
Authors:
K Kobayashi; H P Morris; N Katunuma
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of biochemistry     Volume:  80     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1976 Nov 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  1085-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Carcinoma, Hepatocellular / enzymology*
Leucine / metabolism
Liver / enzymology*
Liver Neoplasms / enzymology*
Male
Neoplasms, Experimental
Ornithine-Oxo-Acid Transaminase / metabolism*
Rats
Transaminases / metabolism*
Chemical
Reg. No./Substance:
61-90-5/Leucine; EC 2.6.1.-/Transaminases; EC 2.6.1.13/Ornithine-Oxo-Acid Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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