Document Detail

Studies on trypsin inhibitors. Part IV, Synthesis of the protected undecapeptide (sequence 25-35) of porcine pancreatic secretory trypsin inhibitor II (Kazal).
MedLine Citation:
PMID:  1270194     Owner:  NLM     Status:  MEDLINE    
Synthesis is described of the protected undecapeptide tert-butyloxycarbonylisoleucyl-threonyltyrosylserylasparaginyl-gamma-tert-butylglutamyl-S-acetamidomethylcysteinyl-valylleucyl-S-acetamidomethylcysteinylseriny hydrazide corresponding to positions 25-35 of the amino acid sequence of porcine pancreatic secretory trypsin inhibitor II (Kazal). The hepatapeptide free base methyl asparaginyl-gamma-tert-butylglutamyl-S-acetamidomethylcysteinylvalylleucyl-S-acetamidomethylcysteinylserinate (sequency 29-35) was acylated, by the azide procedure, with the tetrapeptide tert-butyloxycarbonl-isoleucylthreonyltyrosylserine hydrazide /sequence 25-28) and the resulting tert-butyloxycarbonylundecapeptide methyl ester was transformed into the corresponding hydrazide by hydrazinolysis. The stereochemical homogeneity of the final product was assessed, after partial deprotection with aqueous 90% trifuoroacetic acid, by digestion with papain and aminopeptidase M followed by quantitative amino acid analysis.
A Guggi; R Tomatis; C A Benassi; R Rocchi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of peptide and protein research     Volume:  8     ISSN:  0367-8377     ISO Abbreviation:  Int. J. Pept. Protein Res.     Publication Date:  1976  
Date Detail:
Created Date:  1976-08-03     Completed Date:  1976-08-03     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0330420     Medline TA:  Int J Pept Protein Res     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  97-105     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Amino Acids / analysis
Peptides / analysis,  chemical synthesis*
Trypsin Inhibitor, Kazal Pancreatic / analysis,  chemical synthesis*
Trypsin Inhibitors / chemical synthesis*
Reg. No./Substance:
0/Amino Acids; 0/Peptides; 0/Trypsin Inhibitors; 50936-63-5/Trypsin Inhibitor, Kazal Pancreatic

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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