Document Detail

Studies on thymocyte subpopulations in guinea pigs. III. Physical and functional characterization of six subpopulations separated by density gradient centrifugation and PNA binding.
MedLine Citation:
PMID:  6605265     Owner:  NLM     Status:  MEDLINE    
Thymocytes were separated according to increasing buoyant density into the three subpopulations Ia (25% of recovered cells), Ib (20%) and II (55%), and according to binding to peanut agglutinin (PNA)into PNA+ (65%) and PNA- cells (35%). The frequency of PNA+ was 56% in Ia, 60% in Ib and 66% in population II. Electronic cell volume determinations disclosed mean volumes of 160 fl for Ia, 130 fl for Ib and 100 fl for population II. PNA+ and PNA- cells were very similar as regards cell volume. Thus, PNA+ and PNA- cells are remarkably uniformly distributed among cell categories of different density and cell volume. The rapidly cycling thymocytes, regarded as the most immature cells in the thymus, and the target cells for a thymocyte growth factor both belonged to the PNA+ cells of population Ia. The mitogen-responsive thymocytes also belonged to population Ia, but were PNA-. The largest subpopulation of thymocytes, apparently corresponding to the small, non-cycling cortical cells, were recovered as PNA+ cells of population II.
G Sandberg; O Söder; S Kölare; U Ernström
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell biology     Volume:  51     ISSN:  0304-3568     ISO Abbreviation:  Exp. Cell Biol.     Publication Date:  1983  
Date Detail:
Created Date:  1983-12-20     Completed Date:  1983-12-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7701827     Medline TA:  Exp Cell Biol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  257-66     Citation Subset:  IM    
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MeSH Terms
Cell Separation
Centrifugation, Density Gradient
DNA / biosynthesis
Growth Substances / pharmacology
Guinea Pigs
Lectins / diagnostic use
Lymphocyte Activation
Peanut Agglutinin
T-Lymphocytes / classification*
Thymus Gland / cytology*
Reg. No./Substance:
0/Growth Substances; 0/Lectins; 0/Mitogens; 0/Peanut Agglutinin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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