Document Detail

Studies on the specificity of CNF, a phospholipase A2 inhibitor isolated from the blood plasma of the South American rattlesnake (Crotalus durissus terrificus). I. Interaction with PLA2 from Lachesis muta muta snake venom.
MedLine Citation:
PMID:  10519652     Owner:  NLM     Status:  MEDLINE    
A phospholipase A2 inhibitor has been previously purified and cloned from the blood plasma of the South American rattlesnake, Crotalus durissus terrificus. This inhibitor, named CNF for Crotalus neutralizing factor, interacts with crotoxin, the main neurotoxin from C. d. terrificus venom, abolishing its phospholipase A2 activity. Crotoxin is a heterodimer of an acidic subunit (CA) and a basic phospholipase A2 (CB). CNF acts by forming a stable non-toxic complex with CB, replacing CA in the toxic CA-CB of crotoxin. In the present investigation, we have shown that CNF has a broader specificity. It is able to inhibit the PLA2 activity of the whole venom from the bushmaster snake (Lachesis muta muta), a species evolutionary related to Crotalus. Inhibition experiments have been carried out with four PLA2 active components isolated from L. m. muta venom, one basic and three acidic ones. CNF inhibition is not restricted to the basic PLA2, but extended to the three acidic forms as well.
C L Fortes-Dias; M L Jannotti; F J Franco; A Magalhães; C R Diniz
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  37     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  1999 Dec 
Date Detail:
Created Date:  1999-10-28     Completed Date:  1999-10-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1747-59     Citation Subset:  IM    
Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, Belo Horizonte, Minas Gerais, Brazil.
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MeSH Terms
Chromatography, Ion Exchange
Crotalid Venoms / enzymology*
Crotalus / blood*
Crotoxin / pharmacology
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors / pharmacology*
Glycoproteins / isolation & purification,  pharmacology*
Phospholipases A / antagonists & inhibitors*
Phospholipases A2
Reptilian Proteins*
South America
Species Specificity
Viper Venoms / enzymology
Reg. No./Substance:
0/Crotalid Venoms; 0/Enzyme Inhibitors; 0/Glycoproteins; 0/Reptilian Proteins; 0/Viper Venoms; 0/phospholipase A2 inhibitor protein, Crotalus; 9007-40-3/Crotoxin; EC 3.1.1.-/Phospholipases A; EC A2

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