Document Detail

Studies on rat liver catalase. X. Effect of hemin and an inhibitor on the translation of catalase messenger RNA1.
MedLine Citation:
PMID:  422538     Owner:  NLM     Status:  MEDLINE    
Rat liver catalase mRNA was translated in a rabbit reticulocyte lysates and wheat germ cell-free system in the presence or absence of hemin and/or a translational inhibitor prepared from reticulocytes, liver cells, and wheat germs. Failure to add hemin to the lysates, or the addition of a hemin-regulated translational inhibitor (HRI) to the hemin-supplemented lysates caused a repressed translation. A preparation of inhibitor from rat liver showed activity similar to that of HRI for this translating system. The translation repression by rat liver inhibitor was reversed by eIF-2 (initiation factor) or GTP, but ATP enhanced the repression. The translation of catalase mRNA in the wheat germ system was not affected by the addition of hemin. An inhibitor prepared from wheat germ extracts, as well as the rat liver inhibitor, markedly decreased the rate of translation. eIF-2, GTP, and ATP behaved in the manner described above. Catalase synthesis in a cell-free system derived from rat liver (using endogenous mRNA) was not influenced by either hemin or the inhibitor. The possibilities are discussed that the synthesis of catalase in liver cells is controlled by a translational inhibitor at the level of chain initiation, and that the formation of the inhibitor from its inactive proinhibitor is regulated by the amount of heme.
T Sakamoto; T Higashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  85     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1979 Feb 
Date Detail:
Created Date:  1979-05-26     Completed Date:  1979-05-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  389-96     Citation Subset:  IM    
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MeSH Terms
Adenosine Triphosphate / pharmacology
Catalase / biosynthesis*
Guanosine Triphosphate / pharmacology
Heme / analogs & derivatives*
Hemin / pharmacology*
Liver / enzymology*,  physiology
Plant Physiological Phenomena
Protein Biosynthesis / drug effects*
RNA, Messenger / metabolism*
Reticulocytes / metabolism,  physiology
Triticum / physiology
Reg. No./Substance:
0/RNA, Messenger; 14875-96-8/Heme; 16009-13-5/Hemin; 56-65-5/Adenosine Triphosphate; 86-01-1/Guanosine Triphosphate; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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