| Studies on the purification and characterization of human factor 8. | |
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MedLine Citation:
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PMID: 4626584 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Factor VIII (antihemophilic globulin) has been prepared from Hyland method IV AHG and cryoprecipitate using limited chymotryptic digestion followed by Sepharose gel filtration. The activity of factor VIII is unaffected by the digestion procedure, while fibrinogen in converted to large noncoagulable fragments. The purified factor VIII has been found to be a macromolecular glycoprotein with a major subunit of 240,000, as shown by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Carbohydrate analysis of factor VIII gave values of 1% sialic acid, 2.8% hexosamine, and 1-2% hexose (mannose, galactose, and fucose). The lipid content was found to be less than 5% of the protein content, and included no detectable phospholipid. The amino acid content is also reported. Immunoelectrophoretic analysis using rabbit antibody to purified factor VIII produced a single precipitin line. The chymotrypsin digestion step facilitates the preparation of factor VIII by reducing the viscosity of fibrinogen in the crude starting material, thereby increasing fivefold the quantity of material which can be processed at one time. It also improves markedly the resolution between factor VIII and fibrinogen on gel filtration. |
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Authors:
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S L Marchesi; N R Shulman; H R Gralnick |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 51 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1972 Aug |
Date Detail:
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Created Date: 1972-10-18 Completed Date: 1972-10-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2151-61 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Carbamates Chromatography, Gel Chymotrypsin Cold Temperature Electrophoresis, Disc Factor VIII / analysis, isolation & purification* Fibrinogen Hexoses / analysis Humans Immunodiffusion Immunoelectrophoresis Lipids / analysis Methods |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbamates; 0/Hexoses; 0/Lipids; 9001-27-8/Factor VIII; 9001-32-5/Fibrinogen; EC 3.4.21.1/Chymotrypsin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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