Document Detail


Studies of protein-protein interaction using countercurrent distribution in aqueous two-phase systems. Partition behaviour of six Calvin-cycle enzymes from a crude spinach (Spinacia oleracea) chloroplast extract.
MedLine Citation:
PMID:  2730589     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The partition behaviour of six enzymes of the Calvin cycle in extracts of chloroplasts from spinach (Spinacia oleracea) between two aqueous phases has been studied by countercurrent distribution. The enzymes showed distribution patterns which indicate heterogeneity and the presence of two or three fractions of most of the enzymes. When two of the enzymes, phosphoglycerate kinase and fructose-bisphosphate aldolase, were partitioned in both purified and partially purified form, they behaved like homogeneous substances. These results indicate that countercurrent distribution of crude extracts in aqueous two-phase systems is a useful method to study protein-protein interaction.
Authors:
L O Persson; G Johansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  259     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-07-03     Completed Date:  1989-07-03     Revised Date:  2010-09-10    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  863-70     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Brassica / enzymology
Chloroplasts / enzymology
Countercurrent Distribution*
Enzymes / metabolism*
Pentose Phosphate Pathway*
Solutions
Water
Chemical
Reg. No./Substance:
0/Enzymes; 0/Solutions; 7732-18-5/Water
Comments/Corrections

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