Document Detail


Studies on the profibrinolytic actions of heparin and its fractions.
MedLine Citation:
PMID:  3875899     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Utilizing modified immunochemical methods (ELISA and radioimmunoassay) tissue plasminogen activator, B beta 15-42 RPs and protein C antigen levels were measured in man and a subhuman primate model after subcutaneous and intravenous administration of various low molecular weight heparin fractions. A wide scatter in the data was observed in the t-PA and B beta 15-42 RP levels; however, statistical analysis of the data revealed that certain low molecular weight heparin fractions increased the levels of these endogenous markers of fibrinolysis. No significant alteration in the protein C levels was noted at any time; however, a wide scatter in these data was also evident. The profibrinolytic actions of low molecular weight heparin fractions may be related to the release of t-PA, which is easily measured in plasma. Since it has strong affinity for endogenous sites (thrombus, surface), wide scatter in the data may occur. Physical exercise may also increase the levels. Most of the results reported in our studies represent data on blood samples that were obtained using the simple venipuncture method. We also find that the intravenous administration of the low molecular weight heparin fractions also caused a shortening of the ELT. Since the low molecular weight heparin fractions are heterogeneous in nature, the profibrinolytic actions may be related to one or more of these constituent fragments. Thus, the molecular identity of the profibrinolytic component of low molecular weight fractions remains unknown at this time. Also unknown is if there is a relationship between this effect and anticoagulant activity.
Authors:
J Fareed; J M Walenga; D A Hoppensteadt; H L Messmore
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Seminars in thrombosis and hemostasis     Volume:  11     ISSN:  0094-6176     ISO Abbreviation:  Semin. Thromb. Hemost.     Publication Date:  1985 Apr 
Date Detail:
Created Date:  1985-09-27     Completed Date:  1985-09-27     Revised Date:  2006-03-07    
Medline Journal Info:
Nlm Unique ID:  0431155     Medline TA:  Semin Thromb Hemost     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  199-207     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Enzyme-Linked Immunosorbent Assay
Factor IX / pharmacology
Factor IXa
Fibrin Fibrinogen Degradation Products / metabolism
Fibrinolysis / drug effects*
Glycoproteins / blood
Heparin / pharmacology*
Humans
Macaca mulatta
Male
Peptide Fragments*
Plasminogen Activators / blood
Protein C
Serum Globulins / metabolism
Chemical
Reg. No./Substance:
0/Fibrin Fibrinogen Degradation Products; 0/Glycoproteins; 0/Peptide Fragments; 0/Protein C; 0/Serum Globulins; 0/fibrinogen Bbeta (15-42); 9001-28-9/Factor IX; 9005-49-6/Heparin; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.22/Factor IXa

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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