Document Detail


Studies of the polysaccharide fraction from the lipopolysaccharide of Pseudomonas alcaligenes.
MedLine Citation:
PMID:  4369226     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Studies of the lipopolysaccharide of Pseudomonas alcaligenes strain BR 1/2 were extended to the polysaccharide moiety. The crude polysaccharide, obtained by mild acid hydrolysis of the lipopolysaccharide, was fractionated by gel filtration. The major fraction was the phosphorylated polysaccharide, for which the approximate proportions of residues were; glucose (2), rhamnose (0.7), heptose (2-3), galactosamine (1), alanine (1), 3-deoxy-2-octulonic acid (1), phosphorus (5-6). The heptose was l-glycero-d-manno-heptose. The minor fractions from gel filtration contained free 3-deoxy-2-octulonic acid, P(i) and PP(i). The purified polysaccharide was studied by periodate oxidation, methylation analysis, partial hydrolysis, and dephosphorylation. All the rhamnose and part of the glucose and heptose occur as non-reducing terminal residues. Other glucose residues are 3-substituted, and most heptose residues are esterified with condensed phosphate residues, possibly in the C-4 position. Free heptose and a heptosylglucose were isolated from a partial hydrolysate of the polysaccharide. The location of galactosamine in the polysaccharide was not established, but either the C-3 or C-4 position appears to be substituted and a linkage to alanine was indicated. In its composition, the polysaccharide from Ps. alcaligenes resembles core polysaccharides from other pseudomonads: no possible side-chain polysaccharide was detected.
Authors:
J A Lomax; G W Gray; S G Wilkinson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  139     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1974 Jun 
Date Detail:
Created Date:  1974-10-24     Completed Date:  1974-10-24     Revised Date:  2013-06-12    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  633-43     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alanine / analysis
Alcaligenes / analysis*
Cell Wall / analysis
Chromatography, Gas
Chromatography, Gel
Chromatography, Paper
Chromatography, Thin Layer
Deoxy Sugars / analysis
Diphosphates / analysis
Electrophoresis, Paper
Galactosamine / analysis
Glucose / analysis
Heptoses / analysis
Hydrolysis
Ketoses / analysis
Lipopolysaccharides / analysis*
Methylation
Oxidation-Reduction
Periodic Acid
Phosphates / metabolism
Phosphorus / analysis
Polysaccharides, Bacterial / analysis*
Rhamnose / analysis
Sugar Acids / analysis
Chemical
Reg. No./Substance:
0/Deoxy Sugars; 0/Diphosphates; 0/Heptoses; 0/Ketoses; 0/Lipopolysaccharides; 0/Phosphates; 0/Polysaccharides, Bacterial; 0/Sugar Acids; 10450-60-9/Periodic Acid; 10485-94-6/Rhamnose; 50-99-7/Glucose; 56-41-7/Alanine; 7535-00-4/Galactosamine; 7723-14-0/Phosphorus
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