Document Detail


Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A.
MedLine Citation:
PMID:  11549466     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active.
Authors:
N J Kershaw; M Mukherji; C H MacKinnon; T D Claridge; B Odell; A S Wierzbicki; M D Lloyd; C J Schofield
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  11     ISSN:  0960-894X     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2001-09-10     Completed Date:  2002-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  2545-8     Citation Subset:  IM    
Affiliation:
The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, South Parks Road, OX1 3QY, Oxford, UK.
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MeSH Terms
Descriptor/Qualifier:
Coenzyme A / chemical synthesis*,  chemistry,  metabolism
Humans
Magnetic Resonance Spectroscopy
Mixed Function Oxygenases / chemistry,  genetics,  metabolism*
Phytanic Acid / analogs & derivatives*,  chemical synthesis*,  chemistry,  metabolism
Recombinant Proteins / chemistry,  genetics,  metabolism
Spectrometry, Mass, Electrospray Ionization
Stereoisomerism
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 0/hydroxyphytanoyl-coenzyme A; 0/phytanoyl-coenzyme A; 14721-66-5/Phytanic Acid; 85-61-0/Coenzyme A; EC 1.-/Mixed Function Oxygenases; EC 1.14.-/PHYH protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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