| Studies on phytanoyl-CoA 2-hydroxylase and synthesis of phytanoyl-coenzyme A. | |
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MedLine Citation:
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PMID: 11549466 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Phytanoyl-CoA 2-hydroxylase (PAHX), an iron(II) and 2-oxoglutarate-dependent oxygenase, catalyses an essential step in the mammalian metabolism of beta-methylated fatty acids. Phytanoyl-CoA was synthesised and used to develop in vitro assays for PAHX. The product of the reaction was confirmed as 2-hydroxyphytanoyl-CoA by NMR and mass spectrometric analyses. In accord with in vivo analyses, hydroxylation of both 3R and 3S epimers of the substrate was catalysed by PAHX. Both pro- and mature- forms of PAHX were fully active. |
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Authors:
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N J Kershaw; M Mukherji; C H MacKinnon; T D Claridge; B Odell; A S Wierzbicki; M D Lloyd; C J Schofield |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioorganic & medicinal chemistry letters Volume: 11 ISSN: 0960-894X ISO Abbreviation: Bioorg. Med. Chem. Lett. Publication Date: 2001 Sep |
Date Detail:
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Created Date: 2001-09-10 Completed Date: 2002-03-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9107377 Medline TA: Bioorg Med Chem Lett Country: England |
Other Details:
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Languages: eng Pagination: 2545-8 Citation Subset: IM |
Affiliation:
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The Oxford Centre for Molecular Sciences and The Dyson Perrins Laboratory, South Parks Road, OX1 3QY, Oxford, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Coenzyme A
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chemical synthesis*,
chemistry,
metabolism Humans Magnetic Resonance Spectroscopy Mixed Function Oxygenases / chemistry, genetics, metabolism* Phytanic Acid / analogs & derivatives*, chemical synthesis*, chemistry, metabolism Recombinant Proteins / chemistry, genetics, metabolism Spectrometry, Mass, Electrospray Ionization Stereoisomerism |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins; 0/hydroxyphytanoyl-coenzyme A; 0/phytanoyl-coenzyme A; 14721-66-5/Phytanic Acid; 85-61-0/Coenzyme A; EC 1.-/Mixed Function Oxygenases; EC 1.14.-/PHYH protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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