Document Detail


Studies on phospholipase A2 in human seminal plasma.
MedLine Citation:
PMID:  1612188     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
1. Human seminal plasma and posterior lobe of prostate was found to have phospholipase A2 (PLA2) activity hydrolysing phosphatidylethanolamine with 14C-labelled linoleic and arachidonic acid. 2. A negative relationship was between sperm count and PLA2 activity in human seminal plasma. 3. The purified PLA2 from human seminal plasma showed high affinity to heparin, sensitivity toward p-bromophenacyl bromide, Pb2+, dithioerythritol and EDTA and it was activated by Ca2+ and Mn2+. 4. The purified PLA2 had alkaline pH optimum (7.5-10.0) and pI-value of 5.3. In SDS-PAGE enzyme preparation resulted in two bands with mol. wt of 14,000 and 16,000.
Authors:
S Rönkkö; M Räsänen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  24     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-07-28     Completed Date:  1992-07-28     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  987-92     Citation Subset:  IM    
Affiliation:
Department of Anatomy, University of Kuopio, Finland.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Humans
Male
Molecular Weight
Phospholipases A / chemistry,  isolation & purification,  metabolism*
Phospholipases A2
Prostate / enzymology
Semen / enzymology*
Chemical
Reg. No./Substance:
EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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