| Studies on peptides. CLXVII. Solid-phase syntheses and immunological properties of fragment peptides related to human malaria circumsporozoite protein. | |
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MedLine Citation:
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PMID: 2776243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A glycine-linked tetramer of Asn-Ala-Asn-Pro, a tandem repeated sequence of malaria circumsporozoite (CS) protein, was synthesized by the Boc-based solid phase method, followed by deprotection with 1 M trimethylsilyl trifluoromethanesulfonate-thioanisole in trifluoroacetic acid. In addition, three tetramer-related peptides were similarly synthesized, i.e., a 34-residue peptide [linked with TH, a proposed T-cell epitope of CS, at the C-terminus of the tetramer], a 46-residue peptide and a 59-residue peptide [linked with HA or HA', two proposed T-cell epitopes of influenza hemagglutinin protein, at the N-terminus of the above 34-residue peptide]. Their immunological properties were examined by enzyme-linked immunosorbent assay, for which three different congenic strains of mouse were used to raise the specific antibodies. Despite conjugation of T-cell epitopes to the tetramer, the mice of low-responder strains to the tetramer failed to produce any antibody specific to the tetramer. However, with the aid of recombinant interleukin 2 as an adjuvant, the low-responder mice produced antibody with relatively high titers. |
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Authors:
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K Akaji; Y Hayashi; N Fujii; T Y Liu; I Berkower; H Yajima |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chemical & pharmaceutical bulletin Volume: 37 ISSN: 0009-2363 ISO Abbreviation: Chem. Pharm. Bull. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-10-20 Completed Date: 1989-10-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0377775 Medline TA: Chem Pharm Bull (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 1612-5 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Surface
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chemical synthesis*,
immunology Humans Peptide Fragments / chemical synthesis*, immunology Protozoan Proteins* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Peptide Fragments; 0/Protozoan Proteins; 0/circumsporozoite protein, Protozoan |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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