Document Detail

Studies on nucleotide diphosphate diacylglycerol specificity of acidic phospholipid biosynthesis in rat liver subcellular fractions.
MedLine Citation:
PMID:  949484     Owner:  NLM     Status:  MEDLINE    
(1)Cytidine diphosphate diacylglycerol, uridine diphosphate diacylglycerol, adenosine diphosphate diacylglycerol and guanosine diphosphate diacylglycerol were synthesized chemically and their purity assessed. The acticity of these compounds in acidic phospholipid synthesis was examined in rat liver mitochondria and microsomes. (2) Phosphatidylglycerol synthesis in rat liver mitochondria exhibited considerable activity with CDP diacylglycerol (v 7.0 nmol mg-1 h-1). UDPdiacylglycerol (v 5.4) and ADP diacylglyc erol (v 4.2). GDP diacylglycerol activity was detectable but very low. (3) Diphophatidylglycerol formation in mitochondria and phosphatidylinositolsythesis in microsomes exhibited considerable more specificity for CDP diacylglycerol. However, at high concentrations, measurable diphosphaticylglycerol and phophatidylinositol synthesis was observed with the other liponucleotides. (4) Although considerable phosphatidylglycerol formation was observed with UDPdiacaylglycerol and ADP diacylglycerol, it is unlikelky that these compounds are of physiologic importance, at least in rat liver, since CTP:phophatidic acid cytidyltransferase in microsomes and mitochondria was shown to be specific for cytidine triphosphate. The lack of specificity of phosphatiidylglycerol synthesis for CDP diacylglycerol is currently unexplained but may be of some importance in other tissues or in other organisms.
B J Poorthuis; K Y Hostetler
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  431     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  408-15     Citation Subset:  IM    
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MeSH Terms
Cytidine Diphosphate Diglycerides / metabolism*
Liver / metabolism*
Microsomes, Liver / metabolism
Mitochondria, Liver / metabolism
Nucleoside Diphosphate Sugars / metabolism*
Phosphatidic Acids / metabolism
Phospholipids / biosynthesis*
Subcellular Fractions / metabolism
Transferases / metabolism
Reg. No./Substance:
0/Cytidine Diphosphate Diglycerides; 0/Nucleoside Diphosphate Sugars; 0/Phosphatidic Acids; 0/Phospholipids; EC 2.-/Transferases

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