| Studies of monomeric and homodimeric oxazolo[4,5-b]pyridinium cyanine dyes as fluorescent probes for nucleic acids visualization. | |
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MedLine Citation:
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PMID: 16828165 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The series of recently synthesized monomeric and homodimeric cyanine dyes based on monomethine cyanine chromophore with oxazolo[4,5-b]pyridinium and quinoline end groups [Vassilev A, Deligeorgiev T, Gadjev N, Drexhage K-H. Synthesis of novel monomeric and homodimeric cyanine dyes based on oxazolo[4,5-b]pyridinium and quinolinium end groups for nucleic acid detection, Dyes Pigm 2005;66:135-142] were studied as possible fluorescent probes for nucleic acids detection. Significant fluorescence enhancement and intensity level (quantum yield up to 0.75) was observed for all the dyes in the presence of DNA. The oxazolo[4,5-b]pyridinium cyanines demonstrated high sensitivity as fluorescent stains for post-electrophoretic visualization of nucleic acids in agarose gels upon both VIS and UV transillumination, and the visualized band contained 0.8 ng of dsDNA. |
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Authors:
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V B Kovalska; V P Tokar; M Yu Losytskyy; T Deligeorgiev; A Vassilev; N Gadjev; K-H Drexhage; S M Yarmoluk |
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Publication Detail:
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Type: Journal Article Date: 2006-05-25 |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 68 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 2006 Oct |
Date Detail:
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Created Date: 2006-09-04 Completed Date: 2006-12-22 Revised Date: 2012-08-02 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 155-65 Citation Subset: IM |
Affiliation:
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Institute of Molecular Biology and Genetics, National Academy of Science of Ukraine, 150 Zabolotnogo St., 03143 Kyiv, Ukraine. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbocyanines
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chemistry* DNA / analysis, chemistry Dimerization Electrophoresis, Agar Gel Fluorescent Dyes / chemistry* Indoles / chemistry* Nucleic Acids / analysis*, chemistry Oxazoles / chemistry* Pyridinium Compounds / chemistry* Spectrometry, Fluorescence Staining and Labeling |
| Chemical | |
Reg. No./Substance:
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0/BEBO cyanine dye; 0/Carbocyanines; 0/Fluorescent Dyes; 0/Indoles; 0/Nucleic Acids; 0/Oxazoles; 0/Pyridinium Compounds; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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