Document Detail


Studies of mitochondria and mitochondrial DNA extracted from organelles harboring an intramitochondrial virus.
MedLine Citation:
PMID:  854028     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
(1) Two viper cells lines were investigated, one which harbors IMV in the mitochondria (VSW cells) and one without detectable IMV (VH3 cells). (2) The size of closed circular mtDNA molecules from both VSW and VH3 cells was found to be significantly greater (5.4 to 5.6 micron) than the contour lengths of typical mammalian cells (4.8 to 5.2 micron). (3) A small percentage of mini-circles ranging in size from 0.1 to 0.6 micron was observed to band with closed circular mtDNA from both cell lines. Minicircles were especially abundant in VH3 cells. (4) MtDNA from VSW cells contained 34.1% dimers plus oligomers (10.2% oligomers), whereas VH3 cells had only 14.8% dimeric and oligomeric forms (5.4% oligomers). (5) Treatment of VSW cells with 1 microng/ml ethidium bromide for 48 hours resulted in an increased incidence of IMV (IMV in 15% of mitochondrial sections) as compared with untreated VSW cells (IMV in 3% of mitochondrial sections).
Authors:
W Klietmann; P R Andersen; P D Lunger; H F Clark; M M Nass
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  14     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1977 Feb 
Date Detail:
Created Date:  1977-06-11     Completed Date:  1977-06-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  129-33     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cell Transformation, Neoplastic*
DNA, Circular / metabolism
DNA, Mitochondrial* / metabolism
Microscopy, Electron
Mitochondria / metabolism*
Nucleic Acid Conformation
Chemical
Reg. No./Substance:
0/DNA, Circular; 0/DNA, Mitochondrial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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