| Studies on messenger and ribosomal RNA synthesis in plant tissue cultures induced to undergo synchronus cell division. | |
| | |
MedLine Citation:
|
PMID: 1112268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Messenger and ribosomal RNA metabolism was tudied in a plant tissue culture system: cells from the quiescent tubers of Helianthus tuberosus (Jerusalem artichoke) were induced to divide synchronously and dedeifferentiate by excision and culture of explants in nutrient medium. Large accumulations of ribosomal RNA and protein started early in the 20-h lag-period preceding the first division. In pulse-labelling experiments, two types of polydisperse messenger-like RNAs were detected one with and one without a poly(adenylic acid) sequence. In the first 2 h of culture the two polydisperse RNA fractions were the predominant types of RNA synthesised. Ribosomal RNA synthesis was very low during the first 2 h, but acclerated later. Low concentrations of actinomycin-D strongly inhibited ribosomal RNA synthesis, but had little effect on the synthesis of transfer RNA or either type of polydisperse messenger-like RNA. In explants cultured with low concentrations of actinomycin-D ribosomal RNA accumulation was completely inhibited, but cell division and protein accumulation occurred, though at a reduced rate. It is concluded that the synthesis and accumulation of new ribosomal RNA which normally occur during culture are not required for the induction of cell division or for protein accumulation, i.e. the ribosomal RNA existing in the quiescent tuver cells can support protein accumulation and cell division induced by excision and culture. The quiescent tuber tissue is also shown to contain significant amounts of messenger-like RNA. |
| | |
Authors:
|
R S Fraser |
Related Documents
:
|
22365358 - Characterization of the "viable but nonculturable" (vbnc) state in the wine spoilage ye... 23565178 - The anti-scar effects of basic fibroblast growth factor on the wound repair in vitro an... 3691668 - Oncogene expression in differentiating f9 mouse embryonal carcinoma cells. 6155368 - Studies on antitumor activity of prumycin. iii. mode of action of prumycin on hela s-3 ... 16014738 - In vitro detection of differential and cell-specific hepatobiliary toxicity induced by ... 16666978 - Regulation of embryo dormancy by manipulation of abscisic acid in kernels and associate... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: European journal of biochemistry / FEBS Volume: 50 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1975 Jan |
Date Detail:
|
Created Date: 1975-05-10 Completed Date: 1975-05-10 Revised Date: 2007-07-23 |
Medline Journal Info:
|
Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
|
Languages: eng Pagination: 529-37 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Division Culture Techniques Dactinomycin / pharmacology Plant Proteins / biosynthesis Plants / metabolism* Poly A-U RNA, Messenger / biosynthesis* RNA, Ribosomal / biosynthesis* Time Factors Tritium Ultracentrifugation Uridine / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Ribosomal; 10028-17-8/Tritium; 24936-38-7/Poly A-U; 50-76-0/Dactinomycin; 58-96-8/Uridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: On the quaternary structure of high-molecular-weight proteins from the bovine eye lens.
Next Document: Determination of mRNA half-life in HeLa cultures by a poly(A)-independent direct analysis of specifi...