Document Detail

Studies on messenger and ribosomal RNA synthesis in plant tissue cultures induced to undergo synchronus cell division.
MedLine Citation:
PMID:  1112268     Owner:  NLM     Status:  MEDLINE    
Messenger and ribosomal RNA metabolism was tudied in a plant tissue culture system: cells from the quiescent tubers of Helianthus tuberosus (Jerusalem artichoke) were induced to divide synchronously and dedeifferentiate by excision and culture of explants in nutrient medium. Large accumulations of ribosomal RNA and protein started early in the 20-h lag-period preceding the first division. In pulse-labelling experiments, two types of polydisperse messenger-like RNAs were detected one with and one without a poly(adenylic acid) sequence. In the first 2 h of culture the two polydisperse RNA fractions were the predominant types of RNA synthesised. Ribosomal RNA synthesis was very low during the first 2 h, but acclerated later. Low concentrations of actinomycin-D strongly inhibited ribosomal RNA synthesis, but had little effect on the synthesis of transfer RNA or either type of polydisperse messenger-like RNA. In explants cultured with low concentrations of actinomycin-D ribosomal RNA accumulation was completely inhibited, but cell division and protein accumulation occurred, though at a reduced rate. It is concluded that the synthesis and accumulation of new ribosomal RNA which normally occur during culture are not required for the induction of cell division or for protein accumulation, i.e. the ribosomal RNA existing in the quiescent tuver cells can support protein accumulation and cell division induced by excision and culture. The quiescent tuber tissue is also shown to contain significant amounts of messenger-like RNA.
R S Fraser
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  50     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1975 Jan 
Date Detail:
Created Date:  1975-05-10     Completed Date:  1975-05-10     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  529-37     Citation Subset:  IM    
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MeSH Terms
Cell Division
Culture Techniques
Dactinomycin / pharmacology
Plant Proteins / biosynthesis
Plants / metabolism*
Poly A-U
RNA, Messenger / biosynthesis*
RNA, Ribosomal / biosynthesis*
Time Factors
Uridine / metabolism
Reg. No./Substance:
0/Plant Proteins; 0/RNA, Messenger; 0/RNA, Ribosomal; 10028-17-8/Tritium; 24936-38-7/Poly A-U; 50-76-0/Dactinomycin; 58-96-8/Uridine

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