|Studies on lysosomes. XII. Redistribution of acid hydrolases in human lymphocytes stimulated by phytohemagglutinin.|
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|PMID: 5656399 Owner: NLM Status: MEDLINE|
|Subcellular fractions were isolated by differential centrifugation from pure suspensions of human blood lymphocytes incubated with and without phytohemagglutinin (PHA). Between 30 and 120 min after addition of PHA to intact cells, redistribution of acid hydrolases (beta glucuronidase, acid phosphatase), from a 20,000 g x 20 min granular fraction into the corresponding supernatant, was observed. No increase in total acid hydrolase activity was found at these times. The mitochondrial marker enzyme, malate dehydrogenase, did not undergo redistribution. Granules derived from PHA-treated cells became more fragile upon subsequent incubation with membrane-disruptive agents in vitro (streptolysin S, filipin). These changes were associated with an increase in the over-all permeability of the stimulated cell to substances in the surrounding medium, such as neutral red. Augmentation of dye entry into lymphocytes required intact metabolism as judged by response to temperature and inhibitors (cyanide, antimycin A, 2,4-dinitrophenol). PHA, however, did not release enzyme activity from hydrolase-rich granules in vitro or render them more susceptible to subsequent challenge with membrane-disruptive agents. These studies suggest that PHA induces early changes in the surface of lymphocytes. The consequent redistribution of acid hydrolases may play a role in remodeling processes of the stimulated cells.|
|R Hirschhorn; G Brittinger; K Hirschhorn; G Weissmann|
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|Type: Journal Article|
|Title: The Journal of cell biology Volume: 37 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1968 May|
|Created Date: 1968-08-07 Completed Date: 1968-08-07 Revised Date: 2010-06-22|
Medline Journal Info:
|Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES|
|Languages: eng Pagination: 412-23 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Antimycin A / pharmacology
Cyanides / pharmacology
Dinitrophenols / pharmacology
Glucuronidase / metabolism
Hydrolases / metabolism*
Lectins / pharmacology*
Lymphocytes / drug effects*, enzymology*
Lysosomes / drug effects*
Malate Dehydrogenase / metabolism
Proteins / metabolism
|0/Coloring Agents; 0/Cyanides; 0/Dinitrophenols; 0/Lectins; 0/Proteins; 642-15-9/Antimycin A; EC 220.127.116.11/Malate Dehydrogenase; EC 3.-/Hydrolases; EC 18.104.22.168/Acid Phosphatase; EC 22.214.171.124/Glucuronidase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Journal ID (nlm-ta): J Cell Biol
Publisher: The Rockefeller University Press
Received Day: 24 Month: 10 Year: 1967
Revision Received Day: 10 Month: 1 Year: 1968
Print publication date: Day: 1 Month: 5 Year: 1968
Volume: 37 Issue: 2
First Page: 412 Last Page: 423
PubMed Id: 5656399
|STUDIES ON LYSOSOMES : XII. Redistribution of Acid Hydrolases in Human Lymphocytes Stimulated by Phytohemagglutinin|
|From the Department of Medicine, the New York University School of Medicine, New York 10016 and the Department of Pediatrics, the Mount Sinai School of Medicine, New York
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