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Studies on lysosomes. XII. Redistribution of acid hydrolases in human lymphocytes stimulated by phytohemagglutinin.
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MedLine Citation:
PMID:  5656399     Owner:  NLM     Status:  MEDLINE    
Subcellular fractions were isolated by differential centrifugation from pure suspensions of human blood lymphocytes incubated with and without phytohemagglutinin (PHA). Between 30 and 120 min after addition of PHA to intact cells, redistribution of acid hydrolases (beta glucuronidase, acid phosphatase), from a 20,000 g x 20 min granular fraction into the corresponding supernatant, was observed. No increase in total acid hydrolase activity was found at these times. The mitochondrial marker enzyme, malate dehydrogenase, did not undergo redistribution. Granules derived from PHA-treated cells became more fragile upon subsequent incubation with membrane-disruptive agents in vitro (streptolysin S, filipin). These changes were associated with an increase in the over-all permeability of the stimulated cell to substances in the surrounding medium, such as neutral red. Augmentation of dye entry into lymphocytes required intact metabolism as judged by response to temperature and inhibitors (cyanide, antimycin A, 2,4-dinitrophenol). PHA, however, did not release enzyme activity from hydrolase-rich granules in vitro or render them more susceptible to subsequent challenge with membrane-disruptive agents. These studies suggest that PHA induces early changes in the surface of lymphocytes. The consequent redistribution of acid hydrolases may play a role in remodeling processes of the stimulated cells.
R Hirschhorn; G Brittinger; K Hirschhorn; G Weissmann
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  37     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1968 May 
Date Detail:
Created Date:  1968-08-07     Completed Date:  1968-08-07     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  412-23     Citation Subset:  IM    
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MeSH Terms
Acid Phosphatase / metabolism
Antimycin A / pharmacology
Coloring Agents
Cyanides / pharmacology
Dinitrophenols / pharmacology
Glucuronidase / metabolism
Hydrolases / metabolism*
Lectins / pharmacology*
Lymphocytes / drug effects*,  enzymology*
Lysosomes / drug effects*
Malate Dehydrogenase / metabolism
Proteins / metabolism
Reg. No./Substance:
0/Coloring Agents; 0/Cyanides; 0/Dinitrophenols; 0/Lectins; 0/Proteins; 642-15-9/Antimycin A; EC Dehydrogenase; EC 3.-/Hydrolases; EC Phosphatase; EC

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Received Day: 24 Month: 10 Year: 1967
Revision Received Day: 10 Month: 1 Year: 1968
Print publication date: Day: 1 Month: 5 Year: 1968
Volume: 37 Issue: 2
First Page: 412 Last Page: 423
ID: 2107423
PubMed Id: 5656399

STUDIES ON LYSOSOMES : XII. Redistribution of Acid Hydrolases in Human Lymphocytes Stimulated by Phytohemagglutinin
Rochelle Hirschhorn
Günter Brittinger
Kurt Hirschhorn
Gerald Weissmann
From the Department of Medicine, the New York University School of Medicine, New York 10016 and the Department of Pediatrics, the Mount Sinai School of Medicine, New York

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