Document Detail

Studies of the levamisole inhibitory effect on rat stromal-cell commitment to mineralization.
MedLine Citation:
PMID:  8227184     Owner:  NLM     Status:  MEDLINE    
The ability of Levamisole to decrease mineralization in skeletal tissue is usually related to its effect on alkaline phosphatase (ALP). However, Levamisole is also suspected to diminish mineralization by an additional mechanism which is unrelated to the ALP control of apatite crystal growth. To delineate the time in differentiation during which Levamisole inhibits mineralization, a tissue culture model system of bone marrow stromal cells was used. Secondary cultures of stromal cells were propagated in osteoprogenitor cell (OPC) induction medium for three weeks, followed by measurement of calcium precipitation. In situ ALP assays at pH 7.6 were also performed. When cells were cultured with 0.2 mM Levamisole for three weeks, Day 20 values of calcium precipitates were lower than in controls, but Day 20 ALP values were paradoxically higher. The correlation between calcium and ALP within each group was low. The correlation slightly improved, in uninhibited cultures, when Day 21 calcium values were matched with earlier Day 12 ALP values. This suggested the existence of a Levamisole-sensitive mechanism for mineralization inhibition effective prior to the culture's mineralization stage. To focus on this early effect on mineralization Levamisole was added to stromal cultures on different days and removed on Day 12. Levamisole decreased Day 21 mineralization when added on Days 0, 3, 5, and 7, but not when added on Day 9. The Levamisole-induced inhibition of mineralization was accompanied by an increase in Day 12 ALP specific activity, compared to controls, when added from Day 5 and thereafter. The results indicate that part of the ability of stromal cells to mineralize is determined during the first week of culture.(ABSTRACT TRUNCATED AT 250 WORDS)
B Y Klein; I Gal; D Segal
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  53     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-12-21     Completed Date:  1993-12-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  114-21     Citation Subset:  IM; S    
Laboratory of Experimental Surgery, Hadassah Medical Center, Ein-Kerem, Jerusalem, Israel.
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MeSH Terms
Alkaline Phosphatase / metabolism
Bone and Bones / drug effects,  metabolism*
Calcification, Physiologic / drug effects*
Calcium / metabolism
Cells, Cultured
Chemical Precipitation
Hydrogen-Ion Concentration
Levamisole / pharmacology*
Time Factors
Reg. No./Substance:
14769-73-4/Levamisole; 7440-70-2/Calcium; EC Phosphatase

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