Document Detail

Studies on the intracellular localization of hHR23B.
MedLine Citation:
PMID:  16253613     Owner:  NLM     Status:  MEDLINE    
Yeast Rad23, originally identified as a DNA repair protein, has been proposed to participate in other cellular functions, i.e., the proteasome-degradation pathway, the process of spindle pole body duplication and as a component of the anaphase checkpoint. Two human homologs of yeast Rad23, hHR23A and hHR23B, exhibit high sequence homology with yRad23 and also have been shown to be involved in DNA repair and proteasome-dependent degradation. Previous studies on the intracellular localization of hHR23A and hHR23B revealed their predominant localization in the nucleus during interphase and in the cytoplasm during mitosis. We have analyzed the localization of hHR23B during all the phases of the cell cycle using immunofluorescence. Unlike previous studies, our results suggest localization of hHR23B in the nucleus as well as in the cytoplasm during G1 phase. The nuclear levels of hHR23B decrease during S-phase of the cell cycle. When the cell enters mitosis, hHR23B relocalizes in the cytoplasm without association with chromatin. These results indicate that the intracellular distribution hHR23B is cell cycle dependent.
Samiksha Katiyar; William J Lennarz
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2005-10-10
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  337     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-10-28     Completed Date:  2005-12-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1296-300     Citation Subset:  IM    
Department of Biochemistry and Cell Biology, The Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
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MeSH Terms
Cell Nucleus / metabolism
Cytoplasm / metabolism
DNA Repair Enzymes
DNA-Binding Proteins / metabolism*
G1 Phase
Hela Cells
Protein Transport
S Phase
Grant Support
Reg. No./Substance:
0/DNA-Binding Proteins; 0/RAD23B protein, human; 156533-33-4/RAD23A protein, human; EC 6.5.1.-/DNA Repair Enzymes

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