Document Detail

Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes.
MedLine Citation:
PMID:  12364978     Owner:  NLM     Status:  MEDLINE    
AIM: To study the integration of hepatitis B virus (HBV) DNA into sperm chromosomes in hepatitis B patients and the features of its integration. METHODS: Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm chromosomes. RESULTS: Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis B. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots and the others 2 to 4 signals. The fluorescence intensity showed significant difference among the signal spots. The distribution of signal sites among chromosomes seems to be random. CONCLUSION: HBV could integrate into human sperm chromosomes. Results suggest that the possibility of vertical transmission of HBV via the germ line to the next generation is present.
Jian-Min Huang; Tian-Hua Huang; Huan-Ying Qiu; Xiao-Wu Fang; Tian-Gang Zhuang; Jie-Wen Qiu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Asian journal of andrology     Volume:  4     ISSN:  1008-682X     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-10-04     Completed Date:  2003-03-03     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  209-12     Citation Subset:  IM    
Department of Cell Biology and Medical Genetics, Shantou University Medical College, Shantou 515031, China.
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MeSH Terms
Chromosomes, Human / genetics,  virology
Hepatitis B / genetics,  transmission,  virology*
Hepatitis B virus / genetics*
In Situ Hybridization, Fluorescence
Infectious Disease Transmission, Vertical
Spermatozoa / virology*
Virus Integration*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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