Document Detail

Studies in adipose-derived stromal cells: migration and participation in repair of cranial injury after systemic injection.
MedLine Citation:
PMID:  21364416     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Adipose-derived stromal cells are a multipotent cell type with the ability to undergo osteogenic differentiation. The authors sought to examine whether systemically administered adipose-derived stromal cells would migrate to and heal surgically created defects of the mouse cranial skeleton.
METHODS: Mouse adipose-derived stromal cells were harvested from luciferase-positive transgenic mice; human adipose-derived stromal cells were harvested from human lipoaspirate and labeled with luciferase and green fluorescent protein. A 4-mm calvarial defect (critical sized) was made in the mouse parietal bone; skin incisions alone were used as a control (n = 5 per group). Adipose-derived stromal cells were injected intravenously (200,000 cells per animal) and compared with saline injection only. Methods of analyses included micro-computed tomographic scanning, in vivo imaging system detection of luciferase activity, and standard histology.
RESULTS: Migration of adipose-derived stromal cells to calvarial defect sites was confirmed by accumulation of luciferase activity and green fluorescent protein stain as early as 4 days and persisting up to 4 weeks. Little activity was observed among control groups. Intravenous administration of either mouse or human adipose-derived stromal cells resulted in histologic evidence of bone formation within the defect site, in comparison with an absence of bone among control defects. By micro-computed tomographic analysis, human but not mouse adipose-derived stromal cells stimulated significant osseous healing.
CONCLUSIONS: Intravenously administered adipose-derived stromal cells migrate to sites of calvarial injury. Thereafter, intravenous human adipose-derived stromal cells contribute to bony calvarial repair. Intravenous administration of adipose-derived stromal cells may be an effective delivery method for future efforts in skeletal regeneration.
Benjamin Levi; Aaron W James; Emily R Nelson; Shijun Hu; Ning Sun; Michelle Peng; Joseph Wu; Michael T Longaker
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  127     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-03-02     Completed Date:  2011-04-26     Revised Date:  2014-10-13    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1130-40     Citation Subset:  AIM; IM    
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MeSH Terms
Adipose Tissue / cytology*
Cell Differentiation
Cell Movement*
Cells, Cultured
Disease Models, Animal
Injections, Intravenous
Mice, Nude
Middle Aged
Osteogenesis / physiology*
Parietal Bone / injuries*,  pathology
Stem Cell Transplantation / methods*
Stromal Cells / cytology,  metabolism*
Wound Healing / physiology*
Grant Support
1 R21 DE019274-01/DE/NIDCR NIH HHS; 1F32AR057302-02/AR/NIAMS NIH HHS; R21 DE019274/DE/NIDCR NIH HHS; R21 DE019274-01/DE/NIDCR NIH HHS; RC2 DE020771/DE/NIDCR NIH HHS; RC2 DE020771-01/DE/NIDCR NIH HHS; RC2 DE020771-01/DE/NIDCR NIH HHS

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