Document Detail

Studies on human pure pancreatic juice collected by endoscopic retrograde catheterization of the papilla.
MedLine Citation:
PMID:  729999     Owner:  NLM     Status:  MEDLINE    
Pure pancreatic juice was collected from 8 subjects by endoscopic retrograde cannulation of the papilla. Samples were collected at 1 minute intervals for 20 minutes after rapid intravenous injection of secretin (Eisai, 1u/kg) and for 10 to 15 minutes after rapid intravenous injection of CCK-PZ (1 U/kg). Three hydrolases (amylase, chymotrypsinogen and lipase) showed nonparallel secretory pattern. Lipase was proportionally more stimulated than chymotrypsinogen and chymotrypsinogen was more stimulated than amylase by CCK-PZ stimulation. However, in one subject the secretion of three hydrolases was parallel and in another subject lipase and chymotrypsinogen showed parallelism. Samples collected at "wash out" period showed quite high enzyme and protein concentration with specific activity and enzyme ratio not different from those of samples collected at peak period of CCK-PZ stimulation. Normal ranges were estimated in peak flow rate per minute, peak enzyme concentration, specific activity of enzymes, maximal enzyme output per minute and maximal bicarbonate concentration.
H Harada; A Ono; N Yamamoto; I Ikubo; A Negron; T Hayashi; T Sato; K Mishima; I Kimura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gastroenterologia Japonica     Volume:  13     ISSN:  0435-1339     ISO Abbreviation:  Gastroenterol. Jpn.     Publication Date:  1978  
Date Detail:
Created Date:  1979-03-24     Completed Date:  1979-03-24     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0152744     Medline TA:  Gastroenterol Jpn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  383-9     Citation Subset:  IM    
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MeSH Terms
Amylases / secretion*
Catheterization / methods
Cholecystokinin / pharmacology
Chymotrypsinogen / secretion*
Endoscopy / methods
Lipase / secretion*
Pancreatic Ducts
Pancreatic Juice / enzymology*,  secretion
Specimen Handling / methods*
Reg. No./Substance:
9011-97-6/Cholecystokinin; 9035-75-0/Chymotrypsinogen; EC; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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