Document Detail

Studies on human porin XXII: cell membrane integrated human porin channels are involved in regulatory volume decrease (RVD) of HeLa cells.
MedLine Citation:
PMID:  10870851     Owner:  NLM     Status:  MEDLINE    
Cell volume regulation receives increasing attention not only as the basis of regulatory volume increase or regulatory volume decrease (RVD) of cells in surroundings of changing osmolarity, but also appears to be relevant in cell proliferation, differentiation, and apoptosis. A central event in RVD is the opening of a volume-sensitive chloride/anion channel(s), and blocking this pathway would abolish RVD. This is shown here with monoclonal mouse anti-human type-1 porin antibodies, proving that porin is involved in this process. HeLa cells preincubated with these antibodies dramatically increase their volume within about 1 min after a hypotonic stimulus by 70 mM NaCl Ringer solution, but do not move back toward their starting volume, thus indicating abolished RVD. Corresponding effects are induced by the established anion channel inhibitor DIDS. Video camera monitoring of cell size over time was used as a direct and noninvasive approach. We had already accumulated evidence that plasmalemma integrated eukaryotic porin channels form chloride/anion channels in this cell compartment and that they are involved in cell volume regulation. Finally, the present data again demonstrate the suitability of our anti-porin antibodies in physiological studies.
F P Thinnes; K P Hellmann; T Hellmann; R Merker; U Brockhaus-Pruchniewicz; C Schwarzer; G Walter; H Götz; N Hilschmann
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular genetics and metabolism     Volume:  69     ISSN:  1096-7192     ISO Abbreviation:  Mol. Genet. Metab.     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-08-07     Completed Date:  2000-08-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9805456     Medline TA:  Mol Genet Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  331-7     Citation Subset:  IM    
Abteilung Immunchemie, Max-Planck-Institut für experimentelle Medizin, Göttingen, Germany.
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MeSH Terms
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / pharmacology
Antibodies, Monoclonal / immunology,  pharmacology
Cell Size / drug effects,  physiology
Hela Cells
Ion Channels / antagonists & inhibitors,  physiology
Isotonic Solutions / pharmacology
Membrane Proteins / physiology
Porins / drug effects,  immunology,  physiology*
Video Recording
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ion Channels; 0/Isotonic Solutions; 0/Membrane Proteins; 0/Porins; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 8026-10-6/Ringer's solution

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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