| Studies on human porin XXII: cell membrane integrated human porin channels are involved in regulatory volume decrease (RVD) of HeLa cells. | |
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MedLine Citation:
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PMID: 10870851 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell volume regulation receives increasing attention not only as the basis of regulatory volume increase or regulatory volume decrease (RVD) of cells in surroundings of changing osmolarity, but also appears to be relevant in cell proliferation, differentiation, and apoptosis. A central event in RVD is the opening of a volume-sensitive chloride/anion channel(s), and blocking this pathway would abolish RVD. This is shown here with monoclonal mouse anti-human type-1 porin antibodies, proving that porin is involved in this process. HeLa cells preincubated with these antibodies dramatically increase their volume within about 1 min after a hypotonic stimulus by 70 mM NaCl Ringer solution, but do not move back toward their starting volume, thus indicating abolished RVD. Corresponding effects are induced by the established anion channel inhibitor DIDS. Video camera monitoring of cell size over time was used as a direct and noninvasive approach. We had already accumulated evidence that plasmalemma integrated eukaryotic porin channels form chloride/anion channels in this cell compartment and that they are involved in cell volume regulation. Finally, the present data again demonstrate the suitability of our anti-porin antibodies in physiological studies. |
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Authors:
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F P Thinnes; K P Hellmann; T Hellmann; R Merker; U Brockhaus-Pruchniewicz; C Schwarzer; G Walter; H Götz; N Hilschmann |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Molecular genetics and metabolism Volume: 69 ISSN: 1096-7192 ISO Abbreviation: Mol. Genet. Metab. Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-08-07 Completed Date: 2000-08-07 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9805456 Medline TA: Mol Genet Metab Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 331-7 Citation Subset: IM |
Affiliation:
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Abteilung Immunchemie, Max-Planck-Institut für experimentelle Medizin, Göttingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology Animals Antibodies, Monoclonal / immunology, pharmacology Cell Size / drug effects, physiology Hela Cells Humans Ion Channels / antagonists & inhibitors, physiology Isotonic Solutions / pharmacology Membrane Proteins / physiology Mice Porins / drug effects, immunology, physiology* Video Recording |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Ion Channels; 0/Isotonic Solutions; 0/Membrane Proteins; 0/Porins; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 8026-10-6/Ringer's solution |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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