Document Detail

Studies of host-graft interactions in vitro.
MedLine Citation:
PMID:  17873428     Owner:  NLM     Status:  MEDLINE    
Progenitor and stem cell transplantation represent therapeutic strategies for retinal disorders that are accompanied by photoreceptor degeneration. The transplanted cells may either replace degenerating photoreceptors or secrete beneficial factors that halt the processes of photoreceptor degeneration. The present study analyzes whether rat retinal progenitor cells differentiated into photoreceptor phenotypic cells in neurospheres have a potential to interact with rat retinal explants. Immunocytochemistry for rhodopsin and synaptophysin indicated photoreceptor cell-like differentiation in neurospheres that were stimulated by basic fibroblast growth factor and epidermal growth factor. Differentiation into neural phenotypes including photoreceptor cells was effectively blocked by an addition of leukemia inhibitory factor. Grafting of neurospheres onto retinal explants demonstrated a consistent penetration of glial cell processes into the explanted tissue. On the other hand, the incorporation of donor cells into explants was very low. A general finding was that neurospheres grafting was associated with local decrease in Müller cell activation in the explants. Further characterization of these effect(s) could provide further insight into progenitor cell-based therapies of retinal degenerative disorders.
Ingela Liljekvist-Larsson; Kjell Johansson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-22
Journal Detail:
Title:  Journal of neural engineering     Volume:  4     ISSN:  1741-2560     ISO Abbreviation:  J Neural Eng     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2008-08-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101217933     Medline TA:  J Neural Eng     Country:  England    
Other Details:
Languages:  eng     Pagination:  255-63     Citation Subset:  IM    
Department of Ophthalmology, Lund University, Sweden.
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MeSH Terms
Animals, Newborn
Cell Differentiation
Cells, Cultured
Host vs Graft Reaction / physiology*
Rats, Sprague-Dawley
Retina / cytology*,  physiology,  surgery*
Stem Cell Transplantation / methods*
Stem Cells / cytology*

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