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Studies on the hemolytic properties of protamine.
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MedLine Citation:
PMID:  13688446     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The basic protein protamine causes a rapid hemolysis when incubated with the red blood cells of many mammalian species. The age of the cells does not affect the process. Neutralization of the active side groups of the protamine molecule with formalinization demonstrates that a specific degree of charge is necessary for hemolysis, as more than 30 per cent of the guanidine groups must remain unreacted to maintain activity. Unlike the hemolysis induced by the synthetic polypeptides polylysine and polyhomoarginine, protamine hemolysis is temperature-dependent. Whole lipoprotein material derived from red blood cell membranes inhibits protamine hemolysis to a greater extent than do the membranes themselves, serum, serum protein fractions, or cholesterol. The phosphatide and protein moieties derived from the membranes are quite avid in inhibiting protamine hemolysis. A probable explanation is that intracellular aggregation of these structural elements may cause changes in electrostatic charge and surface tension which result in increased permeability. The hemolytic and antitumor cell properties of protamine could not be segregated from its animal toxicity. Despite formalinization to a degree which eliminated the former, the compound remained quite toxic to mice and rabbits.
Authors:
F F BECKER
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of general physiology     Volume:  44     ISSN:  0022-1295     ISO Abbreviation:  J. Gen. Physiol.     Publication Date:  1961 Jan 
Date Detail:
Created Date:  1961-12-01     Completed Date:  1998-11-01     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  2985110R     Medline TA:  J Gen Physiol     Country:  Not Available    
Other Details:
Languages:  eng     Pagination:  433-42     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Hemolysis*
Protamines / pharmacology*
Chemical
Reg. No./Substance:
0/Protamines
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Gen Physiol
ISSN: 0022-1295
ISSN: 1540-7748
Publisher: The Rockefeller University Press
Article Information
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Copyright © Copyright, 1961, by The Rockefeller Institute
Received Day: 31 Month: 5 Year: 1961
Print publication date: Day: 1 Month: 1 Year: 1961
Volume: 44 Issue: 3
First Page: 433 Last Page: 442
ID: 2195103
PubMed Id: 13688446

Studies on the Hemolytic Properties of Protamine
Frederick F. Becker
From the Department of Pathology, New York University School of Medicine, New York


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