| Studies of four new human myeloma cell lines. | |
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MedLine Citation:
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PMID: 15621787 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe the establishment and studies of four myeloma cell lines that were derived from 2 young individuals with plasma cell leukaemia (Karpas 25 and 1272) and from 2 older persons with multiple myeloma (Karpas 417 and 929). The cultured cells have the ultrastructural appearance of plasma cells with abundant rough endoplasmic reticulum (RER). Their phenotypic profile conform with that of malignant plasma cells. Karyotype analysis revealed that each cell line is unique and all are hyperdipoide with complex aberrant chromosomes. FISH analysis revealed cryptic translocations affecting the IGH locus in 14q32 of 2 of these cell lines. Using R- and G-banding numerous other chromosomal abnormalities were recorded and illustrated in each of the 4 cell lines. |
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Authors:
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Abraham Karpas; Lana Harder; Barbara Czepulkowski; David Bloxham; Rani Saward; Alla Dremucheva; Reiner Siebert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Leukemia & lymphoma Volume: 46 ISSN: 1042-8194 ISO Abbreviation: Leuk. Lymphoma Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2004-12-28 Completed Date: 2005-06-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: England |
Other Details:
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Languages: eng Pagination: 101-12 Citation Subset: IM |
Affiliation:
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Department of Haematology, Cambridge University Clinical School, MRC Centre, UK. karpasa@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor Chromosome Banding Chromosomes, Human / genetics Endoplasmic Reticulum / ultrastructure Humans Immunoglobulins / immunology, metabolism Karyotyping Leukemia, Plasma Cell / genetics*, pathology* Microscopy, Electron Phenotype |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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