| Studies of enzyme reactions in open systems. | |
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MedLine Citation:
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PMID: 7052945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An experimental arrangement is described which allows studies of the temporal performance of enzyme reactions (also reactions of complexes of enzyme chains) under conditions of open systems. The various steady-state properties of such open systems can be recognized by measuring their input and output [1,2]. The various levels of flowing equilibria are perturbed by applying molecular modulators to the system, and the data obtained are compared to those found in closed systems. In order to prove the accuracy of the experimental configuration, the determination of Km in a one-substrate reaction is carried out in the open system, using only one concentration of substrate at various flow rates. |
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Authors:
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B Paletta; R Möller; W Mlekusch; W Truppe |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 2 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 1980 May |
Date Detail:
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Created Date: 1983-08-11 Completed Date: 1983-08-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 283-90 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Enzymes
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metabolism* Kinetics L-Lactate Dehydrogenase / metabolism Lactates / metabolism Pyruvate Kinase / metabolism Pyruvates / metabolism Spectrophotometry / instrumentation, methods |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Lactates; 0/Pyruvates; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 2.7.1.40/Pyruvate Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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