Document Detail

Studies on the effect of glycoprotein processing inhibitors on fusion of L6 myoblast cell lines.
MedLine Citation:
PMID:  2946596     Owner:  NLM     Status:  MEDLINE    
The effect of oligosaccharide processing inhibitors on the fusion of L6 myoblasts was studied. The glucosidase inhibitors, castanospermine, 1-deoxynojirimycin and N-methyl-deoxynojirimycin were potent inhibitors of myoblast fusion, as was the mannosidase II inhibitor, swainsonine. Inhibition of fusion was reversed when inhibitors were removed. However, the mannosidase I inhibitor, 1-deoxymannojirimycin did not inhibit fusion. Changes in cell membrane oligosaccharide structure were followed by monitoring the binding of concanavalin A (conA) and wheat germ agglutinin (WGA) to cell surface membranes in cells treated with processing inhibitors. All the processing inhibitors resulted in increased binding of conA and decreased binding of WGA; this is consistent with the known mechanisms of inhibition of the inhibitors used in the study. Inhibition of fusion by the processing inhibitors also resulted in reduced activities of creatine phosphokinase, an enzyme used as a marker for biochemical differentiation during fusion. Treatment of a non-differentiating conA-resistant cell line with processing inhibitors did not induce fusion, but the cells did show altered lectin-binding properties. The main conclusion drawn from these studies is that cell surface glycoproteins probably containing the mannose (Man)9 structure are important for the fusion reaction.
M A Spearman; J C Jamieson; J A Wright
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  168     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-01-16     Completed Date:  1987-01-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  116-26     Citation Subset:  IM    
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MeSH Terms
Alkaloids / pharmacology
Cell Fusion / drug effects
Cell Line
Concanavalin A / metabolism
Glucosamine / analogs & derivatives,  pharmacology
Glycoproteins / physiology*
Mannose / metabolism
Muscles / cytology*,  drug effects,  metabolism
Oligosaccharides / metabolism
Wheat Germ Agglutinins / metabolism
Reg. No./Substance:
0/Alkaloids; 0/Glycoproteins; 0/Indolizines; 0/Oligosaccharides; 0/Wheat Germ Agglutinins; 11028-71-0/Concanavalin A; 19130-96-2/1-Deoxynojirimycin; 31103-86-3/Mannose; 3416-24-8/Glucosamine; 69567-10-8/N-methyldeoxynojirimycin; 72741-87-8/Swainsonine; 79831-76-8/castanospermine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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