Document Detail

Studies on the deoxyribonucleic acid-bearing portion of the cell envelope of Escherichia coli.
MedLine Citation:
PMID:  366928     Owner:  NLM     Status:  MEDLINE    
The envelope components of nuclear bodies which were obtained from Escherichia coli W7 by a mild lysis method were investigated. By using 2,6-diaminopimelic acid (DAP) as precursor which is incorporated only into peptidoglycan in this strain it was found that the particles contained about 14% of the murein layer of the cell. The percentage of phosphatidylethanolamine was enriched at the cost of the other phospholipids in the nuclear bodies compared to whole cells. If lipids were labelled with 3H-palmitic acid the cytoplasmic and the outer membrane could be found after isopycnic centrifugation; however, when the cells were incubated with chloramphenicol, only the outer membrane was seen. The peptidoglycan and the proteins could be assigned only to the outer membrane. The DNA is also bound to the outer membrane. From these results it was concluded that (1) in all lysis methods the cytoplasmic membrane is more easily dissolved than the outer layers of the envelope, and (2) that there is a firm binding between DNA and the outer membrane in vivo.
V Weisshäupl; P Dworsky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zeitschrift für allgemeine Mikrobiologie     Volume:  18     ISSN:  0044-2208     ISO Abbreviation:  Z. Allg. Mikrobiol.     Publication Date:  1978  
Date Detail:
Created Date:  1979-03-28     Completed Date:  1979-03-28     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  0413631     Medline TA:  Z Allg Mikrobiol     Country:  GERMANY, EAST    
Other Details:
Languages:  eng     Pagination:  681-9     Citation Subset:  IM    
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MeSH Terms
Bacterial Proteins / analysis
Binding Sites
Cell Membrane / analysis,  metabolism
Cell Wall / analysis
Chloramphenicol / pharmacology
Chromosomes, Bacterial / analysis*
DNA, Bacterial / analysis*,  metabolism
Escherichia coli / drug effects,  metabolism,  ultrastructure*
Lipids / analysis
Peptidoglycan / analysis
Phospholipids / analysis
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Bacterial; 0/Lipids; 0/Peptidoglycan; 0/Phospholipids; 56-75-7/Chloramphenicol

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