Document Detail


Studies on the conditions required for optimum recovery of Tetrahymena pyriformis strain S (Phenoset A) after freezing to and thawing from -196 C.
MedLine Citation:
PMID:  807716     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The toxicity of several cryoprotective agents was tested at room temperature (23 C) against Tetrahymena pyriformis strain S (Phenoset A) at different stages of growth cycle. Polyvinylpyrrolidone (PVP-40) at 10 per cent (v/v) concentration was without effect at any stage in the growth cycle, while 1.2 M glycerol immobilized the cells which were disrupted very shortly afterwards. The toxicity of 0.25 M glucose was largely independent of the position of the cells in the growth cycle, but the toxicity of 0.25 M sucrose and 1.4 M dimethylsulfoxide (DMSO) was most marked in late log- and stATIONARY-PHASE CELLS. After log-phase cells had been equilibrated with 1.4 M DMSO for 1 hr, the number of cells surviving cooling at defined rates from 0.45 to 12 C/min decreased as the final temperature decreased from -30 to -60 C. A temperature of -53 C was found to be the optimum from which cells cooled at a given rate could be cooled rapidly to -196 C. Nevertheless, when cells were cooled at defined rates to -35, -45, or -53 C and then rapidly to -196 c the optimum rate of cooling to these temperatures was found to be 1 C/min. The optimum rate of cooling to -60 C prior to plunging into liquid nitrogen was found to be 2.7 C/min.
Authors:
J A Osborne; D Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of protozoology     Volume:  22     ISSN:  0022-3921     ISO Abbreviation:  J. Protozool.     Publication Date:  1975 May 
Date Detail:
Created Date:  1975-11-08     Completed Date:  1975-11-08     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  2985197R     Medline TA:  J Protozool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  233-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cryoprotective Agents / pharmacology
Culture Media
Dimethyl Sulfoxide / pharmacology
Freezing*
Glucose / pharmacology
Glycerol / pharmacology
Povidone / pharmacology
Preservation, Biological*
Sucrose / pharmacology
Temperature
Tetrahymena pyriformis / drug effects,  growth & development*
Chemical
Reg. No./Substance:
0/Cryoprotective Agents; 0/Culture Media; 50-99-7/Glucose; 56-81-5/Glycerol; 57-50-1/Sucrose; 67-68-5/Dimethyl Sulfoxide; 9003-39-8/Povidone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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