Document Detail


Studies of Fe(3)O(4)/Ag/Au composites for immunoassay based on surface plasmon resonance biosensor.
MedLine Citation:
PMID:  23010112     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
A novel method immunosensor based on chemically functionalized Fe(3)O(4)/Ag/Au nanocomposite was developed. Fe(3)O(4)/Ag seeds were coated with Au and Fe(3)O(4)/Ag/Au nanocomposites were obtained. The nanocomposites could be obtained without violent stirring and the operation was easy and convenient. The magnetic nanocomposites can be easily immobilized on Au film of the surface plasmon resonance (SPR) biosensor with a magnetic pillar. The immobilization of Fe(3)O(4)/Ag/Au nanocomposites on the Au film leads to a large shift in resonance wavelength, which is due to the increase of the sensing membrane thickness, high dielectric constant of Ag and Au particles, and electromagnetic coupling between Fe(3)O(4)/Ag/Au nanocomposites and the Au film. The effects of Fe(3)O(4)/Ag/Au nanocomposites on the sensitivity of SPR biosensor were also investigated. As a result, the biosensor based on Au film shows a response for dog IgG in the concentration range of 1.25-20.00μgml(-1), whereas the SPR biosensor based on Fe(3)O(4)/Ag/Au nanocomposites shows a response for dog IgG in the concentration range of 0.15-40.00μgml(-1). The sensitivity of the SPR biosensor based on Fe(3)O(4)/Ag/Au nanocomposites is higher than that based on Au film.
Authors:
Jing Wang; Daqian Song; Hua Zhang; Jia Zhang; Yue Jin; Hanqi Zhang; Hao Zhou; Ying Sun
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-9-5
Journal Detail:
Title:  Colloids and surfaces. B, Biointerfaces     Volume:  102C     ISSN:  1873-4367     ISO Abbreviation:  Colloids Surf B Biointerfaces     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-9-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9315133     Medline TA:  Colloids Surf B Biointerfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  165-170     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, PR China.
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