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Studies on cell deformability. 3. Some effects of EDTA on sarcoma 37 cells.
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MedLine Citation:
PMID:  4962706     Owner:  NLM     Status:  MEDLINE    
The nonlethal procedure of incubation in EDTA solution makes the peripheral regions of ascites sarcoma 37 cells more easily deformable, as reflected in measurements of the decreased amount of negative pressure required to suck out standard hemispherical bulges from the cells into micropipettes. The facilitation of deformability was abolished after reincubation of cells in calcium-containing saline, and this mechanical parameter was partially restored to normal after reincubation in magnesium-containing saline; the mechanical effect of EDTA treatment is, therefore, thought to be due mainly to the removal of calcium from the cell periphery. As EDTA treatment produces no detectable change in cellular electrophoretic mobility, it is concluded that peripheral calcium must be bound to anionic sites deeper than about 10 A from the cellular hydrodynamic slip plane. The data are discussed with emphasis on the view that they should not be extrapolated freely to other cell types.
L Weiss
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  33     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1967 May 
Date Detail:
Created Date:  1967-10-31     Completed Date:  1967-10-31     Revised Date:  2010-06-22    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  341-7     Citation Subset:  IM    
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MeSH Terms
Carbon Isotopes
Chromatography, Thin Layer
Edetic Acid / pharmacology*
Sarcoma 37 / pathology*
Reg. No./Substance:
0/Carbon Isotopes; 60-00-4/Edetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Copyright © 1967 by The Rockefeller University Press
Received Day: 8 Month: 9 Year: 1966
Print publication date: Day: 1 Month: 5 Year: 1967
Volume: 33 Issue: 2
First Page: 341 Last Page: 347
ID: 2108351
PubMed Id: 4962706

STUDIES ON CELL DEFORMABILITY : III. Some Effects of EDTA on Sarcoma 37 Cells
Leonard Weiss
From the Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, New York

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