| Studies on cell-cycle synchronization in the asexual erythrocytic stages of Plasmodium falciparum. | |
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MedLine Citation:
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PMID: 17034650 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Multiplication of Plasmodium parasites within human erythrocytes is essential to malarial disease. The cell-division cycle of this organism, however, is still poorly understood. In other eukaryotes, various techniques for (apparent) cell-cycle synchronization have been used to shed light on the mechanisms involved in cell division and its control. Thus far there is no technique for cell-cycle synchronization (as opposed to selection of parasites of a limited age-range) in Plasmodium. We therefore investigated the possibility that inhibitors of DNA synthesis, the mitotic spindle, or cell-cycle control elements (such as cyclin-dependent kinases) could be used to synchronize P. falciparum cultures to a particular cell-cycle phase. Surprisingly, most of these compounds did not cause a block at a specific phase. Three compounds, Hoechst 33342, roscovitine and L-mimosine, did block development at the trophozoite-schizont transition (S or G2 phase). The block caused by the latter 2 inhibitors was reversible, suggesting that they might be used as synchronizing agents. However, a consideration of the perturbing effects of inhibitors and problems with 'batch' synchronization techniques in general lead us to believe that any results obtained using roscovitine- or L-mimosine-treated parasites may not be reflective of the normal cell cycle. |
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Authors:
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J A Naughton; A Bell |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-11 |
Journal Detail:
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Title: Parasitology Volume: 134 ISSN: 0031-1820 ISO Abbreviation: Parasitology Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-02-01 Completed Date: 2007-04-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401121 Medline TA: Parasitology Country: England |
Other Details:
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Languages: eng Pagination: 331-7 Citation Subset: IM |
Affiliation:
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Department of Microbiology, Moyne Institute of Preventive Medicine, University of Dublin, Trinity College, Dublin 2, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzimidazoles / pharmacology Cell Cycle / drug effects* DNA, Protozoan / biosynthesis, drug effects Erythrocytes / parasitology* Humans Mimosine / pharmacology Parasitology / methods Plasmodium falciparum / cytology*, drug effects, growth & development Purines / pharmacology Schizonts / drug effects, growth & development Trophozoites / drug effects, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Benzimidazoles; 0/DNA, Protozoan; 0/Purines; 0/roscovitine; 23491-52-3/HOE 33342; 500-44-7/Mimosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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