| Studies on carboxymethyl cellulase and xylanase activities of anaerobic fungal isolate CR4 from the bovine rumen. | |
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MedLine Citation:
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PMID: 18791765 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An anaerobic fungal isolate, CR4, was isolated from the bovine rumen. The DNA sequence of internal transcribed spacer region 1 showed that CR4 belonged to the genus Caecocmyces. The dry matter digestibility of timothy hay by anaerobic fungal isolate CR4 was determined. The effects of carbohydrate growth substrates on carboxymethyl cellulase (CMCase) and xylanase activities also were examined. The extent of dry matter digestibility of timothy hay was 31% at 6 days' incubation. The highest specific activity of CMCase in the culture supernatant (SN) fraction was observed in xylose culture. The activity of CMCase was not detected in the SN fraction of cellobiose and xylan or in the cell-bound fraction of all growth substrates. The highest specific activity of xylanase in the SN fraction was observed in glucose culture. These results suggest that fiber-degrading enzyme activities were affected by growth substrates and that CR4 is xylanolytic. Zymogram analysis showed that CR4 produces three CMCases of molecular mass (95, 89, and 64 kDa) and three xylanases of molecular mass (82, 73, and 66 kDa). This is the first demonstration showing the molecular mass of fiber-degrading enzymes of Caecomyces. |
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Authors:
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Hiroki Matsui; Tomomi Ban-Tokuda |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-13 |
Journal Detail:
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Title: Current microbiology Volume: 57 ISSN: 1432-0991 ISO Abbreviation: Curr. Microbiol. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-26 Completed Date: 2009-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 615-9 Citation Subset: IM |
Affiliation:
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Graduate School of Bioresources, Mie University, Mie, Japan. matsui@bio.mie-u.ac.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AB334759 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Animals Cattle Cellulase / chemistry, metabolism* DNA, Fungal / chemistry, genetics DNA, Ribosomal Spacer / genetics Endo-1,4-beta Xylanases / chemistry, metabolism* Fungal Proteins / chemistry, metabolism* Glucose / metabolism Molecular Sequence Data Molecular Weight Neocallimastigales / classification, enzymology*, isolation & purification*, physiology Phleum / metabolism Phylogeny Rumen / microbiology* Sequence Analysis, DNA Xylose / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Fungal; 0/DNA, Ribosomal Spacer; 0/Fungal Proteins; 0/Xylose; 50-99-7/Glucose; EC 3.2.1.4/Cellulase; EC 3.2.1.4/carboxymethylcellulase; EC 3.2.1.8/Endo-1,4-beta Xylanases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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