| Studies of bystander effects in human fibroblasts using a charged particle microbeam. | |
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MedLine Citation:
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PMID: 9881726 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To determine the role of cell-to-cell mediated effects in the response of cells to different radiations by using a charged particle microbeam capable of targeting individual cells with counted particles. MATERIALS AND METHODS: Primary human fibroblasts were irradiated conventionally with X-rays or alpha-particles and scored for the induction of micronucleated or apoptotic cell formation at various times after irradiation. Cells were also individually irradiated with helium-3 particles from a microbeam and the distribution of damaged cells between hit and non-hit cells scored. RESULTS: Conventionally exposed fibroblasts showed a dose-dependent production of micronucleated and apoptotic cells 3 days after irradiation for both X-rays and alpha-particles, with a high RBE value for alpha-particles at low doses. Targeting individual alpha-particles, using a microbeam, to four cells within a population produced more micronucleated and apoptotic cells than expected on the basis of a direct effect only. CONCLUSIONS: This study provides direct evidence for the production of transmissible, cell-to-cell effects between hit and non-hit cells individually exposed to charged particles. |
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Authors:
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K M Prise; O V Belyakov; M Folkard; B D Michael |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of radiation biology Volume: 74 ISSN: 0955-3002 ISO Abbreviation: Int. J. Radiat. Biol. Publication Date: 1998 Dec |
Date Detail:
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Created Date: 1999-01-22 Completed Date: 1999-01-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8809243 Medline TA: Int J Radiat Biol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 793-8 Citation Subset: IM; S |
Affiliation:
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Gray Laboratory Cancer Research Trust, Mount Vernon Hospital, Northwood, Middlesex, UK. Michael@graylab.ac.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alpha Particles
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adverse effects Apoptosis / radiation effects Dose-Response Relationship, Radiation Fibroblasts / radiation effects* Humans Micronuclei, Chromosome-Defective / radiation effects Radiation, Ionizing* X-Rays / adverse effects |
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