Document Detail


Studies on bovine cervical mucin. I. The amino acid composition and N-terminal amino acid of bovine oestrus cervical mucin.
MedLine Citation:
PMID:  19999714     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bovine oestrus cervical mucin, isolated by gel filtration on Sepharose 6B, was found to be homogeneous (i) in electrophoresis on cellulose acetate at pH 8.6 after release of sialic acid and in acrylamide-agarose gel at pH 8.7 in the presence of sodium dodecyl sulphate, where not a single compact zone was found after staining for proteins, (ii) by analytical ultracentrifugation and equilibrium centrifugation in a CsCl density gradient; as well as (iii) by immunoelectrophoresis, which revealed two kinds of antibodies against mucin, reacting, respectively, with the primary structure and with determinants depending on the integrity of the glycoprotein structure. Alanine was identified as the N-terminal amino acid of the peptide core, which appeared to consist of a repetition of a 32-35 residue sequence, viz. (Thr)8, (Glu, Pro, Ala, Val)3, (Asp, Leu)2 and (Arg, Cys, Ileu)1. No integral numbers could be assigned to (Ser)3.4, (Gly)2.3 and (Phe)0.6 which were also present.
Authors:
K S Bhushana Rao; E Van Roost; P L Masson; J F Heremans; F Andre
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  317     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1973 Aug 
Date Detail:
Created Date:  2009-12-15     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  286-302     Citation Subset:  IM    
Affiliation:
Department of Experimental Medicine and Physiology of Human Reproduction Research Unit, University of Louvain, Avenue Chapelle-aux-Champs 4, B-1200, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Acrylamides / chemistry
Amino Acids / chemistry*
Animals
Cattle
Cervix Uteri / metabolism*
Cesium / chemistry
Chlorides / chemistry
Cross-Linking Reagents / chemistry
Estrus / metabolism*
Female
Hydrogen-Ion Concentration
Mucins / chemistry*
Peptide Hydrolases / chemistry
Protein Structure, Tertiary
Sepharose / chemistry
Ultracentrifugation / methods
Chemical
Reg. No./Substance:
0/Acrylamides; 0/Amino Acids; 0/Chlorides; 0/Cross-Linking Reagents; 0/Mucins; 7440-46-2/Cesium; 7647-17-8/cesium chloride; 9012-36-6/Sepharose; EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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