| Studies on bovine cervical mucin. I. The amino acid composition and N-terminal amino acid of bovine oestrus cervical mucin. | |
| | |
MedLine Citation:
|
PMID: 19999714 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bovine oestrus cervical mucin, isolated by gel filtration on Sepharose 6B, was found to be homogeneous (i) in electrophoresis on cellulose acetate at pH 8.6 after release of sialic acid and in acrylamide-agarose gel at pH 8.7 in the presence of sodium dodecyl sulphate, where not a single compact zone was found after staining for proteins, (ii) by analytical ultracentrifugation and equilibrium centrifugation in a CsCl density gradient; as well as (iii) by immunoelectrophoresis, which revealed two kinds of antibodies against mucin, reacting, respectively, with the primary structure and with determinants depending on the integrity of the glycoprotein structure. Alanine was identified as the N-terminal amino acid of the peptide core, which appeared to consist of a repetition of a 32-35 residue sequence, viz. (Thr)8, (Glu, Pro, Ala, Val)3, (Asp, Leu)2 and (Arg, Cys, Ileu)1. No integral numbers could be assigned to (Ser)3.4, (Gly)2.3 and (Phe)0.6 which were also present. |
| | |
Authors:
|
K S Bhushana Rao; E Van Roost; P L Masson; J F Heremans; F Andre |
Related Documents
:
|
6163734 - Isolation of a low molecular weight constituent from kentucky blue grass pollen possess... 6724844 - The photodynamic effect of chlorpromazine, promazine, and hematoporphyrin on lens protein. 6790534 - Isolation and characterization of rat alpha-1-antitrypsin. 6350264 - Purification and characterization of a bacteriocin from bacteroides fragilis. 9174264 - Isolation and quantitation of isotopically labeled amino acids from biological samples. 18164954 - Hydrolysis of miscanthus for bioethanol production using dilute acid presoaking combine... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 317 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1973 Aug |
Date Detail:
|
Created Date: 2009-12-15 Completed Date: 2010-01-07 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 286-302 Citation Subset: IM |
Affiliation:
|
Department of Experimental Medicine and Physiology of Human Reproduction Research Unit, University of Louvain, Avenue Chapelle-aux-Champs 4, B-1200, Belgium. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acrylamides
/
chemistry Amino Acids / chemistry* Animals Cattle Cervix Uteri / metabolism* Cesium / chemistry Chlorides / chemistry Cross-Linking Reagents / chemistry Estrus / metabolism* Female Hydrogen-Ion Concentration Mucins / chemistry* Peptide Hydrolases / chemistry Protein Structure, Tertiary Sepharose / chemistry Ultracentrifugation / methods |
| Chemical | |
Reg. No./Substance:
|
0/Acrylamides; 0/Amino Acids; 0/Chlorides; 0/Cross-Linking Reagents; 0/Mucins; 7440-46-2/Cesium; 7647-17-8/cesium chloride; 9012-36-6/Sepharose; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The chain composition of tetanus toxin.
Next Document: The polypeptide composition of epidermal prekeratin.