| Studies on the anticandidal mode of action of Allium sativum (garlic). | |
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MedLine Citation:
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PMID: 3076177 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mode of action of aqueous garlic extract (AGE) was studied in Candida albicans. The minimum inhibitory concentration (MIC) of AGE against six clinical yeast isolates ranged between 0.8 and 1.6 mg ml-1. Scanning electron microscopy and cell leakage studies showed that garlic treatment affected the structure and integrity of the outer surface of the yeast cells. Growth of C. albicans in the presence of AGE affected the yeast lipid in a number of ways: the total lipid content was decreased; garlic-grown yeasts had a higher level of phosphatidylserines and a lower level of phosphatidylcholines; in addition to free sterols and sterol esters, C. albicans accumulated esterified steryl glycosides; the concentration of palmitic acid (16:0) and oleic acid (18:1) increased and that of linoleic acid (18:2) and linolenic acid (18:3) decreased. Oxygen consumption of AGE-treated C. albicans was also reduced. The anticandidal activity of AGE was antagonized by thiols such as L-cysteine, glutathione and 2-mercaptoethanol. Interaction studies between AGE and thiols included growth antagonism, enzymic inhibition and interference of two linear zones of inhibition. All three approaches suggest that AGE exerts its effect by the oxidation of thiol groups present in the essential proteins, causing inactivation of enzymes and subsequent microbial growth inhibition. |
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Authors:
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M A Ghannoum |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of general microbiology Volume: 134 ISSN: 0022-1287 ISO Abbreviation: J. Gen. Microbiol. Publication Date: 1988 Nov |
Date Detail:
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Created Date: 1989-09-11 Completed Date: 1989-09-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375371 Medline TA: J Gen Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2917-24 Citation Subset: IM |
Affiliation:
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Department of Botany and Microbiology, Faculty of Science, Kuwait University, Safat. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Candida albicans
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growth & development,
metabolism*,
ultrastructure Garlic* Lipids / analysis Oxygen Consumption Plant Extracts / pharmacology Plants, Medicinal* Sulfhydryl Compounds / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Plant Extracts; 0/Sulfhydryl Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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