| Studies on the activities, kinetic properties and subcellular localisation of cyclic AMP phosphodiesterase in human neutrophil leukocytes. | |
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MedLine Citation:
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PMID: 6245817 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A one-step radioassay for cyclic AMP phosphodiesterase was optimised for human polymorphonuclear leukocytes. Kinetic studies indicated the presence of two forms of phosphodiesterase activity with apparent Km values of 0.015 mmol/l and 0.98 mmol/l for cyclic AMP. Control neutrophils were homogenised in isotonic sucrose and, after low speed centrifugation, the supernatant was subjected to analytical subcellular fractionation. Gradient fractions were assayed for principal marker enzymes and for cyclic AMP phosphodiesterase. Both forms of phosphodiesterase activity were located in the cytosol. Polymorphonuclear leukocyte homogenates were isolated from control subjects, patients with chronic granulocytic leukaemia and patients in the third trimester of pregnancy. A portion of each homogenate was used for enzyme analysis and the remainder assayed for cyclic AMP content. The specific activity (mUnits/mg protein) of the low Km phosphodiesterase was reduced in both patient groups compared with control values, whilst that of the high Km phosphodiesterase was unchanged. Leukocytes from patients with chronic granulocytic leukaemia had only a fifth of the cyclic AMP content of control neutrophils, whilst leukocytes from patients in the third trimester of pregnancy had an elevated cyclic AMP content. |
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Authors:
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G P Smith; T J Peters |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 103 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1980 Apr |
Date Detail:
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Created Date: 1980-07-22 Completed Date: 1980-07-22 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 193-201 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3',5'-Cyclic-AMP Phosphodiesterases
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blood* Adult Centrifugation, Density Gradient Cyclic AMP Cytosol / enzymology Female Humans Isoenzymes / blood Kinetics Leukemia, Myeloid / enzymology* Magnesium Male Methods Neutrophils / enzymology* Pregnancy Pregnancy Trimester, Third* Tritium |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 10028-17-8/Tritium; 60-92-4/Cyclic AMP; 7439-95-4/Magnesium; EC 3.1.4.17/3',5'-Cyclic-AMP Phosphodiesterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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