Document Detail


The Studies on the Transfection of EGFP-Fused Porcine Circovirus Type 2 Genome.
MedLine Citation:
PMID:  21502729     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The study described the construction and transfection of EGFP-fused Porcine Circovirus Type 2 (PCV2) genome and the recovery of the virus. Post-transfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK, and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nucleus of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. The presence of ORF2-EGFP fusion protein was demonstrated by dual signals of green fluorescence and anti-PCV2 antibodies conjugated with rhodamine in IFA. Furthermore, the released EGFP-fused PCV2 genome was demonstrated by real-time PCR.
Authors:
Hsu-Chung Gabriel Chen; Shu-Hsiang Yang; Shiow-Suey Lai; Tsun-Yung Kuo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-19
Journal Detail:
Title:  The Journal of veterinary medical science / the Japanese Society of Veterinary Science     Volume:  -     ISSN:  0916-7250     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-19     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105360     Medline TA:  J Vet Med Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
School of Veterinary Medicine, National Taiwan University.
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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