| Studies on Streptococcus pyogenes. III. The effect of trypsin and a cationic detergent on the structure, permeability, and metabolism of the cell. | |
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MedLine Citation:
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PMID: 13463269 Owner: NLM Status: OLDMEDLINE |
Abstract/OtherAbstract:
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Electron microscopic observations of Streptococcus pyogenes (strain S43, type 6) treated with trypsin and cetyltrimethylammonium bromide (Cetab) are presented. The concentration of trypsin necessary to remove the M protein antigen was shown to bring about a partial digestion of the cells. The cell protoplasm and protoplasmic membrane were affected. No microscopic changes appeared in the cell wall. Cetab did not alter the appearance of the cells. Both trypsin and detergent altered the permeability of the cell so that citrulline and carbamylphosphate were metabolized. Cells exhibiting enzyme activity on these substrates after Cetab treatment were characterized by the release of lysine, glutamic acid, nucleic acid, and other cellular material. Enzymes responsible for the metabolism of arginine, citrulline, and carbamylphosphate were shown to reside in the cell protoplasm. Cells which had lost their viability after Cetab treatment still possessed the ability to utilize the latter substrates. Under similar conditions the metabolism of glucose did not occur. Normal cells were shown to possess the ability to fluoresce in the presence of a dye. The intensity of the fluorescence was reduced by trypsin treatment. |
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Authors:
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H D SLADE |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of general physiology Volume: 41 ISSN: 0022-1295 ISO Abbreviation: J. Gen. Physiol. Publication Date: 1957 Sep |
Date Detail:
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Created Date: 1958-12-01 Completed Date: 2000-07-01 Revised Date: 2010-09-20 |
Medline Journal Info:
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Nlm Unique ID: 2985110R Medline TA: J Gen Physiol Country: Not Available |
Other Details:
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Languages: eng Pagination: 63-76 Citation Subset: OM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Streptococcus
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drug effects* |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Gen Physiol ISSN: 0022-1295 ISSN: 1540-7748 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © Copyright, 1957, by The Rockefeller Institute for Medical Research Received Day: 6 Month: 3 Year: 1957 Print publication date: Day: 20 Month: 9 Year: 1957 Volume: 41 Issue: 1 First Page: 63 Last Page: 76 ID: 2194815 PubMed Id: 13463269 |
| STUDIES ON STREPTOCOCCUS PYOGENES : III. THE EFFECT OF TRYPSIN AND A CATIONIC DETERGENT ON THE STRUCTURE, PERMEABILITY, AND METABOLISM OF THE CELL | |
| Hutton D. Slade | |
| From The Rheumatic Fever Research Institute, Chicago |
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