Document Detail

Studies on Shigella boydii infection in Caenorhabditis elegans and bioinformatics analysis of immune regulatory protein interactions.
MedLine Citation:
PMID:  22841995     Owner:  NLM     Status:  Publisher    
Shigella boydii causes bacillary dysentery or shigellosis and generates a significant burden in the developing nations. S. boydii-mediated infection assays were performed at both physiological and molecular levels using C. elegans as a host. Continuous exposure of worms to S. boydii showed a reduced life span indicating the pathogenicity of Shigella. Quantitative Real-Time PCR analysis was performed to analyze the expression and regulation of host specific candidate-antimicrobial genes (clec-60, clec-80, lys-7) and were expressed significantly during early infection, but weakened during the latter hours. Increased mortality of mutant RB1285 by S. boydii and S. flexneri indicated the role of lys-7 during Shigella infection. Protein-Protein interactions (PPIs) database was used to analyze the interaction of immune proteins in both C. elegans and human. In addition, the expression and regulation were revealed about immune genes (clec-61, clec-62, clec-63, F54D5.3 and ZK1320.2), which encode several intermediate immune protein partners (CLEC-61, CLEC-62, CLEC-63, F54D5.3, ZK1320.2, W03D2.6 and THN-2) that interact with LYS-7, CLEC-60 and were found to play a role in C. elegans immune defense against S. boydii infections. Similarly, the immune genes that are specific to the human defense system, which encode IGHVA-39, A2M, LTF, CD79A, were predicted to be expressed with LYZ, MBL2, thus indicating their regulation during Shigella infections. Our results using the lowest eukaryotic model system and human database indicated that the major players involved in immunity-related processes appear to be common in cases of Shigella sp. mediated immune responses. This article is part of a Special Issue entitled: Computational Methods for Protein Interaction and Structural Prediction.
Periyanaina Kesika; Krishnaswamy Balamurugan
Related Documents :
8198585 - Plant nitrate reductase gene fragments enhance nitrite production in activated murine m...
362945 - Macrophage fusion factor elicited from bgg-sensitized lymphocytes.
22300085 - Focus on ulcerative colitis: stable gastric pentadecapeptide bpc 157.
16926395 - Brucella melitensis triggers time-dependent modulation of apoptosis and down-regulation...
22577075 - Pde4 inhibition suppresses il-17-associated immunity in dry eye disease.
23275605 - Thymic stromal lymphopoietin amplifies the differentiation of alternatively activated m...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-26
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  -     ISSN:  0006-3002     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Age-related differences in the neural correlates of remembering time-based intentions.
Next Document:  Compatible solutes contribute to heat resistance and ribosome stability in Escherichia coli AW1.7.