| Studies on a Novel Gelatin Sponge: Preparation and Characterization of Cross-Linked Gelatin Scaffolds Using 2-Chloro-1-Methylpyridinium Iodide as a Zero-Length Cross-Linker. | |
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MedLine Citation:
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PMID: 21549037 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We prepared a novel porous gelatin (GEL) sponge which was cross-linked (CL) with a zero-length cross-linker of 2-chloro-1-methylpyridinium iodide (CMPI), and compared CPMI with 1-ethyl-3,3-dimethylaminoproplycarbodiimide (EDC). The ninhydrin assay indicated that the CMPI-CL-GEL sponge had a higher degree of cross-linking than the EDC-CL-GEL sponge at cross-linking saturation. In contrast, the EDC-CL-GEL sponge demonstrated poor water uptake and a much slower enzymatic degradation rate than the CMPI-CL-GEL sponge. Scanning electron microscopy (SEM) images of the gelatin sponge fabricated using a gradient frozen-lyophilization method showed uniformly distributed and interconnected pores. Human 3T3 fibroblasts were successfully seeded onto the scaffolds, and cell proliferation was sustained on all CL-GEL sponges. CMPI-CL-GEL sponges demonstrated significantly increased cell numbers after day 1, and cell numbers steadily rose from day 1 to 12. Meanwhile, the CMPI-CL-GEL sponge had a higher cell number than the EDC-CL-GEL sponge (P<0.05) by day 4. In vitro studies with 3T3 fibroblasts demonstrated an increased cell viability for those cells grown on sponges cross-linked with CMPI compared to those cross-linked with EDC. SEM images revealed attachment and spreading of cells, the CMPI-CL-GEL sponges had more cells that had elongated, migrated, and formed interconnected networks with neighboring cells. |
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Authors:
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Ming-Kung Yeh; Yan-Ming Liang; Chieh-Shen Hu; Kuang-Ming Cheng; Yao-Wen Hung; Jenn-Jong Young; Po-da Hong |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-3 |
Journal Detail:
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Title: Journal of biomaterials science. Polymer edition Volume: - ISSN: 1568-5624 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007393 Medline TA: J Biomater Sci Polym Ed Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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