Document Detail


Student-designed enzyme-linked metabolite assay kits.
MedLine Citation:
PMID:  21706748     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The extensive use of commercial kits in molecular biology and biochemistry has prompted us to design a series of practical sessions to help students become familiar with the uses and limitations of pre-packaged assay systems. To facilitate an understanding of these assay systems and to promote reflection on their appropriate use, students manufacture their own kit for one of four enzyme-linked metabolite assays. To do this they must investigate the role of each of the components in the assay, optimize the conditions where possible, check for cross reactivity, and then work this assay up for a few related "real" samples. Students make up all the buffers, the standard solution, instructions, and even the packaging. The kit is checked for accuracy by the producers, "marketed" to other students, and evaluated by their peers. By going through this process, students learn the benefits and pitfalls of commercial kits as well as reinforcing the basic principles of metabolite measurement and gaining experience with assay design, troubleshooting, and problem solving.
Authors:
D Hancock; J Johnston; J Dimauro; G Denyer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology     Volume:  32     ISSN:  1470-8175     ISO Abbreviation:  Biochem Mol Biol Educ     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2011-06-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100970605     Medline TA:  Biochem Mol Biol Educ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  326-30     Citation Subset:  -    
Copyright Information:
Copyright © 2004 International Union of Biochemistry and Molecular Biology, Inc.
Affiliation:
University of Sydney, Darlington NSW 2006, Sydney, Australia.
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