Document Detail


Structures of the sugar chains of interferon-gamma produced by human myelomonocyte cell line HBL-38.
MedLine Citation:
PMID:  2503497     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interferon-gamma produced by the human myelomonocyte cell line HBL-38 contained galactose, mannose, fucose, N-acetylglucosamine, and N-acetylneuraminic acid as sugar components. Sugar chains were liberated from interferon-gamma by hydrazinolysis. Free amino groups of the sugar chains were acetylated and the reducing-end sugar residues were tagged with 2-aminopyridine under new reaction conditions in which no sialic acid residue was hydrolyzed. The pyridylamino (PA-) derivatives of the sugar chains thus obtained were purified by gel filtration and reversed-phase HPLC. Seven major PA-sugar chains were isolated and the structure of each purified PA-sugar chain was identified by stepwise exoglycosidase digestion and 500-mHz 1H-NMR spectroscopy. The results indicated that the structures of the major PA-sugar chains were of the biantennary type, to which 0 to 2 mol of fucose and 1 to 2 mol of N-acetylneuraminic acid were linked as shown below. (formula; see text)
Authors:
S Yamamoto; S Hase; S Fukuda; O Sano; T Ikenaka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemistry     Volume:  105     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1989 Apr 
Date Detail:
Created Date:  1989-09-14     Completed Date:  1989-09-14     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  547-55     Citation Subset:  IM    
Affiliation:
Department of Chemistry, College of Science, Osaka University.
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MeSH Terms
Descriptor/Qualifier:
Asialoglycoproteins / analysis
Carbohydrate Sequence
Carbohydrates / analysis*
Cell Line
Chromatography, DEAE-Cellulose
Chromatography, Gel
Chromatography, High Pressure Liquid
Interferon-gamma / analysis*,  isolation & purification
Magnetic Resonance Spectroscopy
Methylation
Molecular Sequence Data
Monocytes / metabolism*
Chemical
Reg. No./Substance:
0/Asialoglycoproteins; 0/Carbohydrates; 82115-62-6/Interferon-gamma

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