| Structures of the sugar chains of interferon-gamma produced by human myelomonocyte cell line HBL-38. | |
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MedLine Citation:
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PMID: 2503497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interferon-gamma produced by the human myelomonocyte cell line HBL-38 contained galactose, mannose, fucose, N-acetylglucosamine, and N-acetylneuraminic acid as sugar components. Sugar chains were liberated from interferon-gamma by hydrazinolysis. Free amino groups of the sugar chains were acetylated and the reducing-end sugar residues were tagged with 2-aminopyridine under new reaction conditions in which no sialic acid residue was hydrolyzed. The pyridylamino (PA-) derivatives of the sugar chains thus obtained were purified by gel filtration and reversed-phase HPLC. Seven major PA-sugar chains were isolated and the structure of each purified PA-sugar chain was identified by stepwise exoglycosidase digestion and 500-mHz 1H-NMR spectroscopy. The results indicated that the structures of the major PA-sugar chains were of the biantennary type, to which 0 to 2 mol of fucose and 1 to 2 mol of N-acetylneuraminic acid were linked as shown below. (formula; see text) |
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Authors:
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S Yamamoto; S Hase; S Fukuda; O Sano; T Ikenaka |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemistry Volume: 105 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1989 Apr |
Date Detail:
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Created Date: 1989-09-14 Completed Date: 1989-09-14 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 547-55 Citation Subset: IM |
Affiliation:
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Department of Chemistry, College of Science, Osaka University. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Asialoglycoproteins
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analysis Carbohydrate Sequence Carbohydrates / analysis* Cell Line Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, High Pressure Liquid Interferon-gamma / analysis*, isolation & purification Magnetic Resonance Spectroscopy Methylation Molecular Sequence Data Monocytes / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Asialoglycoproteins; 0/Carbohydrates; 82115-62-6/Interferon-gamma |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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