Document Detail

Structures of oxaliplatin-oligonucleotide adducts from DNA.
MedLine Citation:
PMID:  23019159     Owner:  NLM     Status:  In-Data-Review    
Oxaliplatin, [(1R,2R)-cyclohexane-1,2-diamine](ethanedioato-O,O')platinum(II) shows a great efficiency against colorectal cancer. Although the mode of action of oxaliplatin is not yet understood, it is commonly accepted that binding of oxaliplatin to DNA prevents DNA synthesis and alters protein to DNA binding. In order to elucidate the modified DNA-protein interaction and thus to understand the mechanisms leading to cellular misinterpretation of DNA information and apoptosis, we have identified the preferential binding sites and the dynamics of the oxaliplatin-DNA intrastrand and interstrand adducts at the oligomer level using high-performance liquid chromatography/electrospray ionization-tandem mass spectrometry (HPLC/ESI-MS/MS) and HPLC/inductively coupled plasma-MS for quantitative studies. We used a combination of benzonase, alkaline phosphatase and Nuclease S1 for digestion. This digestion procedure allows the study of platinated oligomeric nucleotides and more complex interstrand adducts. The digestion products were mostly chromatographically separated and characterized using HPLC/ESI-ion trap MS/MS experiments. We could show that the adducts to guanine and adenine are quite dynamic; that is, the ratios are changing for several days. In addition, the resulting adducts provide evidence for the action of the digesting enzymes and indicate that the adduct spectrum at the oligomeric level is different to that at the commonly studies dinucleotide level. Copyright © 2012 John Wiley & Sons, Ltd.
Shereen Mowaka; Matthias Ziehe; Dalia Mohamed; Ulrike Hochkirch; Jürgen Thomale; Michael W Linscheid
Related Documents :
17562679 - Recent natural hybridization between two allopolyploid wheatgrasses (elytrigia, poaceae...
23019159 - Structures of oxaliplatin-oligonucleotide adducts from dna.
23103339 - Quantitative snp analysis in secondary-structured dna by affinity capillary electrophor...
333389 - Molecular cloning of extensive sequences of the in vitro synthesized chicken ovalbumin ...
23153639 - On-line capillary electrophoresis for enhanced detection sensitivity of feline panleuko...
2549089 - Comparison of in situ hybridization and monoclonal antibodies for early detection of cy...
3522919 - Gene conversion in escherichia coli. resolution of heteroallelic mismatched nucleotides...
19161839 - Pablo analysis of rna: 5'-phosphorylation state and 5'-end mapping.
10852809 - Gene-gun-mediated transfer of reporter genes to somatic zebrafish (danio rerio) tissues.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of mass spectrometry : JMS     Volume:  47     ISSN:  1096-9888     ISO Abbreviation:  J Mass Spectrom     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9504818     Medline TA:  J Mass Spectrom     Country:  England    
Other Details:
Languages:  eng     Pagination:  1282-93     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Humboldt-Universitaet zu Berlin, Department of Chemistry, Berlin, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structural determination of glycopeptidolipids of Mycobacterium smegmatis by high-resolution multipl...
Next Document:  In-situ enrichment of phosphopeptides on MALDI plates modified by ambient ion landing.